A novel click lysine zwitterionic stationary phase for hydrophilic interaction liquid chromatography

被引：46

作者：

Guo, Hongyue ^{[1
]}

Liu, Renhua ^{[1
]}

Yang, Jinjin ^{[1
]}

Yang, Bingcheng ^{[1
]}

Liang, Xinmiao ^{[1
]}

Chu, Changhu ^{[1
]}

机构：

[1] E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2012年 / 1223卷

基金：

中国国家自然科学基金;

关键词：

Click lysine; Analysis; HILIC; beta-Lactam antibiotics; Cephalosporins; Carbapenems; BETA-LACTAM ANTIBIOTICS; MOLECULARLY IMPRINTED POLYMER; HUMAN PLASMA; REVERSED-PHASE; N-GLYCOPEPTIDES; SEPARATION; HPLC; IDENTIFICATION; GLYCOSYLATION; ENRICHMENT;

D O I：

10.1016/j.chroma.2011.12.033

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel type of zwitterionic HILIC stationary phase was prepared by covalently bonding the L-azido lysine on silica gel via click chemistry. The key intermediate azido lysine was synthesized by transformation the amino group in L-Boc-lysine to corresponding azido group and subsequent removal of the N-protected group (Boc). Finally, the azido lysine was covalently bonded to silica beads by click chemistry to get click lysine. Its structure was confirmed by FT-IR and elemental analysis. The new stationary phase showed good HILIC characteristics, when it was applied to separate polar and hydrophilic compounds, such as organic acids, cephalosporins and carbapenems. Compared with the commercial stationary phases, such as Atlantics HILIC and ZIC-HILIC, click lysine displayed better or similar chromatographic behaviors. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：47 / 52

页数：6

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