A convenient method for the synthesis of oligonucleotide-cationic peptide conjugates

被引：1
|
作者：
Ferenc, G [1 ]
Kupihár, Z [1 ]
Kele, Z [1 ]
Kovács, L [1 ]
机构：
[1] Univ Szeged, Dept Med Chem, H-6720 Szeged, Hungary
来源：
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2005年 / 24卷 / 5-7期
关键词：
conjugation; disulfide bond formation; cationic peptide; penetration; oligonucleotide;
D O I：
10.1081/NCN-200060066
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A method was developed for the synthesis of oligonucleolide-cationic peptide conjugates in solution phase by disulfide bond formation. Precipitation was avoided by the easily removable triethylammonium trifluoroacetate (TEATFAc) salt which served at the same time as a buffer of the reaction mixture. The fast and high yielding disulfide bond formation was due to the Npys thio protecting and activating group of Cys. A solution of the free 5'-thiol modified oligonucleotide obtained from Poly-Pak (TM) purification was used for conjugation.
引用
收藏
页码：1059 / 1061
页数：3
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