Tumor Suppressor MicroRNA-493 Decreases Cell Motility and Migration Ability in Human Bladder Cancer Cells by Downregulating RhoC and FZD4

被引:93
作者
Ueno, Koji [1 ]
Hirata, Hiroshi [1 ]
Majid, Shahana [1 ]
Yamamura, Soichiro [1 ]
Shahryari, Varahram [1 ]
Tabatabai, Z. Laura [2 ]
Hinoda, Yuji [4 ]
Dahiya, Rajvir [1 ,3 ]
机构
[1] San Francisco VA Med Ctr, Dept Urol, San Francisco, CA USA
[2] San Francisco VA Med Ctr, Dept Pathol, San Francisco, CA USA
[3] Univ Calif San Francisco, San Francisco, CA 94143 USA
[4] Yamaguchi Univ, Grad Sch Med, Dept Oncol & Lab Med, Ube, Yamaguchi 755, Japan
关键词
HEPATOCELLULAR-CARCINOMA; GENOMIC ANALYSIS; IN-VITRO; METASTASIS; INVASION; PATHWAY; PROGRESSION; INHIBITION; BIOMARKERS; ACTIVATION;
D O I
10.1158/1535-7163.MCT-11-0592
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The purpose of this study was to identify new tumor suppressor microRNAs (miRNA; miR) in bladder cancer, conduct functional analysis of their suppressive role, and identify their specific target genes. To explore tumor suppressor miRs in bladder cancer, miR microarray was conducted using SV-HUC-1, T24, J82, and TCCSUP cells. Expression of miR-493 in bladder cancer (T24, J82, and TCCSUP) cells was downregulated compared with normal SV-HUC-1 cells. Also, the expression of miR-493 was significantly lower in bladder cancer tissues than in their corresponding noncancerous tissues. Transfection of miR-493 into T24 or J82 cells decreased their cell growth andmigration abilities. On the basis of this result, to identify potential miR-493 target genes, we used target scan algorithms to identify target oncogenes related to invasion and migration. miR-493 decreased 30-untranslated region luciferase activity and protein expression of FZD4 and RhoC. miR-493 also decreased binding of RhoC and Rock-1. miR-493 is a new tumor suppressor miRNA in bladder cancer and inhibits cell motility through downregulation of RhoC and FZD4. Mol Cancer Ther; 11(1); 244-53. (C) 2011 AACR.
引用
收藏
页码:244 / 253
页数:10
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