Tumor Suppressor MicroRNA-493 Decreases Cell Motility and Migration Ability in Human Bladder Cancer Cells by Downregulating RhoC and FZD4

被引：93

作者：

Ueno, Koji ^{[1
]}

Hirata, Hiroshi ^{[1
]}

Majid, Shahana ^{[1
]}

Yamamura, Soichiro ^{[1
]}

Shahryari, Varahram ^{[1
]}

Tabatabai, Z. Laura ^{[2
]}

Hinoda, Yuji ^{[4
]}

Dahiya, Rajvir ^{[1
,3
]}

机构：

[1] San Francisco VA Med Ctr, Dept Urol, San Francisco, CA USA

[2] San Francisco VA Med Ctr, Dept Pathol, San Francisco, CA USA

[3] Univ Calif San Francisco, San Francisco, CA 94143 USA

[4] Yamaguchi Univ, Grad Sch Med, Dept Oncol & Lab Med, Ube, Yamaguchi 755, Japan

来源：

MOLECULAR CANCER THERAPEUTICS | 2012年 / 11卷 / 01期

关键词：

HEPATOCELLULAR-CARCINOMA; GENOMIC ANALYSIS; IN-VITRO; METASTASIS; INVASION; PATHWAY; PROGRESSION; INHIBITION; BIOMARKERS; ACTIVATION;

D O I：

10.1158/1535-7163.MCT-11-0592

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The purpose of this study was to identify new tumor suppressor microRNAs (miRNA; miR) in bladder cancer, conduct functional analysis of their suppressive role, and identify their specific target genes. To explore tumor suppressor miRs in bladder cancer, miR microarray was conducted using SV-HUC-1, T24, J82, and TCCSUP cells. Expression of miR-493 in bladder cancer (T24, J82, and TCCSUP) cells was downregulated compared with normal SV-HUC-1 cells. Also, the expression of miR-493 was significantly lower in bladder cancer tissues than in their corresponding noncancerous tissues. Transfection of miR-493 into T24 or J82 cells decreased their cell growth andmigration abilities. On the basis of this result, to identify potential miR-493 target genes, we used target scan algorithms to identify target oncogenes related to invasion and migration. miR-493 decreased 30-untranslated region luciferase activity and protein expression of FZD4 and RhoC. miR-493 also decreased binding of RhoC and Rock-1. miR-493 is a new tumor suppressor miRNA in bladder cancer and inhibits cell motility through downregulation of RhoC and FZD4. Mol Cancer Ther; 11(1); 244-53. (C) 2011 AACR.

引用

页码：244 / 253

页数：10

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