Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain

被引：6
作者：
Gao, Yuhua [1 ,2 ]
Li, Xiangchen [1 ,2 ]
Zheng, Dong [2 ]
Guan, Weijun [1 ]
Ma, Yuehui [1 ]
机构：
[1] Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China
[2] Northeast Forestry Univ, Coll Wildlife Resources, Harbin 150040, Peoples R China
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2015年 / 16卷 / 03期
关键词：
SUBVENTRICULAR ZONE; EGF; FOREBRAIN; DEFECTS; NEURONS; FGF; CNS;
D O I：
10.3390/ijms16035990
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We recently isolated stem cells derived from the brain of a bovine fetus, utilizing a particular mechanical separation method. After improving our experimental conditions, we obtained neural stem cells using an optimized culture medium system. The cells were expanded, established in continuous cell culture and used for immunofluorescence cytochemistry. RT-PCR showed that embryonic neural stem cells (NSCs) not only expresses the protein Sox2, Nestin but also Pax6, Musashi proteins and were differentiated into the three classical neuronal phenotypes (neurons, astrocytes, and oligodendrocytes).
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收藏
页码：5990 / 5999
页数：10
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