IDENTIFICATION OF CUCUMBER MOSAIC VIRUS (CMV) ON SQUASH (CUCURBITA PEPO L.) CULTIVARS IN LAKES REGION OF TURKEY

被引：0
|
作者：
Yardimci, Nejla [1 ]
Kilic, Handan Culal [1 ]
Kor, Aynur [1 ]
机构：
[1] Suleyman Demirel Univ, Fac Agr, Dept Plant Protect, TR-32260 Isparta, Turkey
来源：
FRESENIUS ENVIRONMENTAL BULLETIN | 2015年 / 24卷 / 02期
关键词：
Cucurbits; Cucumber mosaic virus; ELISA; RT-PCR; dsRNA; NECROTIC RINGSPOT VIRUS; MOLECULAR HYBRIDIZATION; MOVEMENT; PLANT; RNA;
D O I：
暂无
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Lakes Region is an important area in cucurbit production of Turkey. During surveys conducted in the 20112012 growing season, field-grown squash plants with symptoms of mosaic, chlorotic mottling, vein banding, blistering and leaf malformation were observed. A total of 268 squash samples were collected from various locations in this region. Cucumber mosaic virus (CMV), was detected in squash, using enzyme-linked immunosorbent assay (ELISA), double stranded RNA (dsRNA) analysis and Reverse transcription polymerase chain reaction (RT-PCR) methods. ELISA tests showed that among 268 field samples tested, 54 were infected with CMV. ELISA-negative samples were tested by RT-PCR for CMV. All samples were positive for CMV. As a result of dsRNA analysis, specific 3 CMV RNA profiles were obtained.
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页码：417 / 421
页数：5
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