Applications of MALDI-TOF technology in clinical microbiology

被引:10
作者
Suarez, S. [1 ]
Nassif, X. [1 ]
Ferroni, A. [1 ]
机构
[1] Hop Necker Enfants Malad, AP HP, Microbiol Lab, F-75015 Paris, France
来源
PATHOLOGIE BIOLOGIE | 2015年 / 63卷 / 01期
关键词
Mass spectrometry; Matrix-Assisted Laser Desorption; Ionization-Time Of Flight (MALDI-TOF); Microbiology; Microbial identification; LASER-DESORPTION IONIZATION; FLIGHT MASS-SPECTROMETRY; RESISTANT STAPHYLOCOCCUS-AUREUS; BLOOD CULTURE BOTTLES; RAPID IDENTIFICATION; ESCHERICHIA-COLI; DESORPTION/IONIZATION-TIME; STREPTOCOCCUS-PNEUMONIAE; MICROORGANISM IDENTIFICATION; ROUTINE IDENTIFICATION;
D O I
10.1016/j.patbio.2014.10.002
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Until now, the identification of micro-organisms has been based on the cultural and biochemical characteristics of bacterial and fungal species. Recently, Mass Spectrometry type Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF MS) was developed in clinical microbiology laboratories. This new technology allows identification of micro-organisms directly from colonies of bacteria and fungi within few minutes. In addition, it can be used to identify germs directly from positive blood culture bottles or directly from urine samples. Other ways are being explored to expand the use of MALDI-TOF in clinical microbiology laboratories. Indeed, some studies propose to detect bacterial antibiotic resistance while others compare strains within species for faster strain typing. The main objective of this review is to update data from the recent literature for different applications of MALDI-TOF technique in microbiological diagnostic routine. (C) 2014 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:43 / 52
页数:10
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