A comparison of kombucha SCOBY bacterial cellulose purification methods

被引：57

作者：

Amarasekara, Ananda S. ^{[1
]}

Wang, Deping ^{[1
]}

Grady, Tony L. ^{[1
]}

机构：

[1] Prairie View A&M Univ, Dept Chem, Prairie View, TX 77446 USA

来源：

SN APPLIED SCIENCES | 2020年 / 2卷 / 02期

基金：

美国国家科学基金会;

关键词：

Kombucha; Scoby; Bacterial cellulose; Hydrogen peroxide; Sodium hypochlorite; FERMENTATION; COMPOSITES; MEMBRANES;

D O I：

10.1007/s42452-020-1982-2

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Kombucha SCOBY pellicle formed as a waste product in the kombucha tea fermentation industry can be used as a potential source for bacterial cellulose. We have compared four simple, scalable purification methods for the purification of this jelly like cellulosic matrix containing bacteria, yeast cells, proteins and polyphenols as impurities. The method using two successive 1.0 M NaOH washings at 90 degrees C, followed by the treatment with 1.5% (w/w) aq. NaOCl for 2 h, was the most effective method and gave the purified cellulose sample with the highest leucometer whiteness value of 81.4 +/- 4.8. The purified cellulose samples were analyzed using FT-IR, TGA, SEM, EDX, X-ray and water absorption at room temperature.

引用

页数：7

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