Activation of yeast protein kinase C by Rho1 GTPase

被引：257
作者：
Kamada, Y
Qadota, H
Python, CP
Anraku, Y
Ohya, Y
Levin, DE
机构：
[1] JOHNS HOPKINS UNIV, SCH PUBL HLTH, DEPT BIOCHEM, BALTIMORE, MD 21205 USA
[2] UNIV TOKYO, GRAD SCH SCI, DEPT BIOL SCI, TOKYO 113, JAPAN
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 1996年 / 271卷 / 16期
关键词：
D O I：
10.1074/jbc.271.16.9193
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have investigated the role of the essential Rho1 GTPase in cell integrity signaling in budding yeast. Conditional rho1 mutants display a cell lysis defect that is similar to that of mutants in the cell integrity signaling pathway mediated by protein kinase C (Pkc1), which is suppressed by overexpression of Pkc1. rho1 mutants are also impaired in pathway activation in response to growth at elevated temperature. Pkc1 co-immunoprecipitates with Rho1 in yeast extracts, and recombinant Rho1 associates with Pkc1 in vitro in a GTP-dependent manner. Recombinant Rho1 confers upon Pkc1 the ability to be stimulated by phosphatidylserine. indicating that Rho1 controls signal transmission through Pkc1.
引用
收藏
页码：9193 / 9196
页数：4
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