A new primer for 16S rDNA analysis of microbial communities associated with Porphyra yezoensis

被引：10
|
作者：
Namba, Aki [1 ,3 ]
Shigenobu, Yuya [1 ]
Kobayashi, Masahiro [2 ]
Kobayashi, Takanori [1 ]
Oohara, Ichiro [1 ]
机构：
[1] Fisheries Res Agcy, Natl Res Inst Fisheries Sci, Aquat Genom Res Ctr, Kanazawa Ku, Kanagawa 2368648, Japan
[2] Fisheries Res Agcy, Res Ctr Fisheries & Environm Ariake & Yatsushiro, Seikai Natl Fisheries Res Inst, Nagasaki 8512213, Japan
[3] Nihon Univ, Lab Fish Pathol, Dept Vet Med, Kanagawa 2520880, Japan
来源：
FISHERIES SCIENCE | 2010年 / 76卷 / 05期
关键词：
Porphyra yezoensis; 16S rDNA; Polymerase chain reaction; Universal primer; Microbial community;
D O I：
10.1007/s12562-010-0273-z
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
To construct high-quality 16S rDNA clone libraries for microbial communities associated with Porphyra yezoensis and to minimize the detection of rDNA from leafy gametophytes of P. yezoensis, we designed a new 16S rDNA universal primer (75F). Of the clones prepared using 75F, which was designed to distinguish between bacteria and P. yezoensis, 95% were classified into four groups, namely, beta-proteobacteria, gamma-proteobacteria, Lentisphaerae, and Flavobacteria. PCR-based analysis of the 16S rDNA primer constructed in this study can be used to implement 16S rDNA-based methodologies for the investigation of microbial community composition and diversity related to the Porphyra group.
引用
收藏
页码：873 / 878
页数：6
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