Foot-and-mouth disease virus induces lysosomal degradation of host protein kinase PKR by 3C proteinase to facilitate virus replication

被引：42

作者：

Li, Chuntian ^{[1
]}

Zhu, Zixiang ^{[1
]}

Du, Xiaoli ^{[1
]}

Cao, Weijun ^{[1
]}

Yang, Fan ^{[1
]}

Zhang, Xiangle ^{[1
]}

Feng, Huanhuan ^{[1
]}

Li, Dan ^{[1
]}

Zhang, Keshan ^{[1
]}

Liu, Xiangtao ^{[1
]}

Zheng, Haixue ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, State Key Lab Vet Etiol Biol, Natl Foot & Mouth Dis Reference Lab, Key Lab Anim Virol,Minist Agr,Lanzhou Vet Res Ins, Lanzhou, Gansu, Peoples R China

来源：

VIROLOGY | 2017年 / 509卷

关键词：

Foot-and-mouth disease virus; PKR; 3C(pro); Lysosomes; DOUBLE-STRANDED-RNA; TRANSLATION INITIATION-FACTOR; LEADER PROTEINASE; INFECTED-CELLS; NS1; PROTEIN; INHIBITION; INTERFERON; ACTIVATION; CLEAVAGE; SITE;

D O I：

10.1016/j.virol.2017.06.023

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The interferon-induced double-strand RNA activated protein kinase (PKR) plays important roles in host defense against viral infection. Here we demonstrate the significant antiviral role of PKR against foot-and-mouth disease virus (FMDV) and report that FMDV infection inhibits PKR expression and activation in porcine kidney (PK-15) cells. The viral nonstructural protein 3 C proteinase (3C(pro)) is identified to be responsible for this inhibition. However, it is independent of the well-known proteinase activity of 3C(Pro) or 3C(Pro)-induced shutoff of host protein synthesis. We show that 3C(Pro) induces PKR degradation by lysosomal pathway and no interaction is determined between 3C(Pro) and PKR. Together, our results indicate that PKR acts an important antiviral factor during FMDV infection, and FMDV has evolved a strategy to overcome PKR-mediated antiviral role by downregulation of PKR protein.

引用

收藏

页码：222 / 231

页数：10

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