Purification, molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus)

被引：20

作者：

Wang, Yajun ^{[2
]}

Osatomi, Kiyoshi ^{[1
]}

Nagatomo, Yoshimi ^{[1
]}

Yoshida, Asami ^{[2
]}

Hara, Kenji ^{[1
]}

机构：

[1] Nagasaki Univ, Fac Fisheries, Nagasaki 8528521, Japan

[2] Nagasaki Univ, Grad Sch Sci & Technol, Nagasaki 8528521, Japan

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2011年 / 158卷 / 04期

关键词：

Manganese-superoxide dismutase; Japanese flounder; Hepatopancreas; Purification; Amino acid sequence; cDNA sequence; Mitochondria-targeting signal; AMINO-ACID-SEQUENCE; OXIDATIVE STRESS; IN-VIVO; EXPRESSION; LIVER; POLYMORPHISMS; MITOCHONDRIA; DEFENSES; COPPER;

D O I：

10.1016/j.cbpb.2010.12.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Manganese-superoxide dismutase (Mn-SOD) from Japanese flounder (Paralichthys olivaceus) hepatopancreas has been purified with high purification (781-fold) and recovery (10.8%). The molecular mass of the purified enzyme was estimated to be 26 kDa by SDS-PAGE under reducing conditions. In activity staining by native-PAGE, the Japanese flounder Mn-SOD gave three active bands and exhibited KCN-insensitive activity. In addition, the electrophoretic mobility of this enzyme was observed to be faster than that of Japanese flounder Cu,Zn-SOD. On the other hand, the N-terminal amino acid sequence of this Mn-SOD was determined to be 16 amino acid residues, and the sequence showed high homology to other Mn-SODs but not Japanese flounder Cu,Zn-SOD. Analysis of nucleotide and deduced amino acid sequences revealed that the Mn-SOD cDNA consisted of a 64 bp 5'-non-coding region, a 675 bp open reading frame encoding 225 amino acids, and a 465 bp 3'-non-coding region. The first 27 amino acids containing a mitochondria-targeting signal were highly conserved among other Mn-SODs. (c) 2011 Elsevier Inc. All rights reserved.

引用

页码：289 / 296

页数：8

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