Genomic Characterization of β-Glucuronidase-Positive Escherichia coli O157:H7 Producing Stx2a

被引：16

作者：

Ogura, Yoshitoshi ^{[1
]}

Seto, Kazuko ^{[2
]}

Morimoto, Yo ^{[3
]}

Nakamura, Keiji ^{[1
]}

Sato, Mitsuhiko P. ^{[1
]}

Gotoh, Yasuhiro ^{[1
]}

Itoh, Takehiko ^{[4
]}

Toyoda, Atsushi ^{[5
]}

Ohnishi, Makoto ^{[6
]}

Hayashi, Tetsuya ^{[1
]}

机构：

[1] Kyushu Univ, Fukuoka, Fukuoka, Japan

[2] Osaka Inst Publ Hlth, Osaka, Japan

[3] Hokkaido Inst Publ Hlth, Sapporo, Hokkaido, Japan

[4] Tokyo Inst Technol, Tokyo, Japan

[5] Natl Inst Genet, Shizuoka, Japan

[6] Natl Inst Infect Dis, Tokyo, Japan

来源：

EMERGING INFECTIOUS DISEASES | 2018年 / 24卷 / 12期

基金：

日本学术振兴会;

关键词：

SHIGA TOXIN 2; SEQUENCE-ANALYSIS; CLONAL STRUCTURE; O157-H7; STRAIN; PATHOGENICITY; ANNOTATION; EXPRESSION; OUTBREAK; PHAGES;

D O I：

10.3201/eid2412.180404

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Among Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 strains, those producing Stx2a cause more severe diseases. Atypical STEC O157:H7 strains showing a beta-glucuronidase-positive phenotype (GP STEC O157:H7) have rarely been isolated from humans, mostly from persons with asymptomatic or mild infections; Stx2a-producing strains have not been reported. We isolated, from a patient with bloody diarrhea, a GP STEC O157:H7 strain (PV15-279) that produces Stx2a in addition to Stx1a and Stx2c. Genomic comparison with other STEC O157 strains revealed that PV15-279 recently emerged from the stx1a/stx2c-positive GP STEC O157:H7 clone circulating in Japan. Major virulence genes are shared between typical (beta-glucuronidase-negative) and GP STEC O157:H7 strains, and the Stx2-producing ability of PV15-279 is comparable to that of typical STEC O157:H7 strains; therefore, PV15-279 presents a virulence potential similar to that of typical STEC O157:H7. This study reveals the importance of GP O157:H7 as a source of highly pathogenic STEC clones.

引用

页码：2219 / 2227

页数：9

共 48 条

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