Antiproliferative activity of peptides prepared from enzymatic hydrolysates of tuna dark muscle on human breast cancer cell line MCF-7

被引：123

作者：

Hsu, Kuo-Chiang ^{[2
]}

Li-Chan, Eunice C. Y. ^{[3
]}

Jao, Chia-Ling ^{[1
]}

机构：

[1] Tung Fang Design Univ, Dept Food Sci & Technol, Kaohsiung 82941, Taiwan

[2] China Med Univ, Dept Nutr, Taichung 40402, Taiwan

[3] Univ British Columbia, Fac Land & Food Syst, Food Nutr & Hlth Program, Vancouver, BC V6T 1Z4, Canada

来源：

FOOD CHEMISTRY | 2011年 / 126卷 / 02期

关键词：

Tuna dark muscle; Antiproliferative activity; Peptide; Protein hydrolysate; PROTEIN HYDROLYSATE; ANCHOVY SAUCE; COOKING JUICE; LUNASIN; ACETYLATION; ANTIOXIDANT; CHOLESTEROL; INHIBITION; ANTICANCER; APOPTOSIS;

D O I：

10.1016/j.foodchem.2010.11.066

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

To produce and identify antiproliferative peptides, two commercial enzymes, papain (PA) and Protease XXIII (PR) were used to hydrolyse tuna dark muscle byproduct, and the protein hydrolysates were purified, before being evaluated for antiproliferative activities against human breast cancer cell line MCF-7. The results showed that the peptide fractions with the molecular weight ranging from 390 to 1400 Da possessed the greatest antiproliferative activity. The amino acid sequences of the two antiproliferative peptides isolated from PA and PR hydrolysates were Leu-Pro-His-Val-Leu-Thr-Pro-Glu-Ala-Gly-Ala-Thr (1206 Da) and Pro-Thr-Ala-Glu-Gly-Gly-Val-Tyr-Met-Val-Thr (1124 Da), whilst they show the dose-dependent inhibition effect of the MCF-7 cells with IC50 values of 8.1 and 8.8 mu M, respectively. We thus conclude that antiproliferative hydrolysates from tuna dark muscle byproduct may be useful ingredients in food and nutraceutical applications. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：617 / 622

页数：6

共 30 条

[1]

Armstrong WB, 2000, CANCER EPIDEM BIOMAR, V9, P43

[2]

Beak HH, 1995, J FOOD SCI, V60, P929

[3] Protein hydrolysates from Pacific whiting solid wastes [J].