The three-dimensional structure of Drosophila melanogaster (6-4) photolyase at room temperature

被引：8

作者：

Cellini, Andrea ^{[1
]}

Wahlgren, Weixiao Yuan ^{[1
]}

Henry, Leocadie ^{[1
]}

Pandey, Suraj ^{[2
]}

Ghosh, Swagatha ^{[1
]}

Castillon, Leticia ^{[1
]}

Claesson, Elin ^{[1
]}

Takala, Heikki ^{[3
,4
]}

Kubel, Joachim ^{[1
]}

Nimmrich, Amke ^{[1
]}

Kuznetsova, Valentyna ^{[3
]}

Nango, Eriko ^{[5
,6
,7
,8
]}

Iwata, So ^{[5
,6
,7
]}

Owada, Shigeki ^{[5
,6
,7
]}

Stojkovic, Emina A. ^{[9
]}

Schmidt, Marius ^{[2
]}

Ihalainen, Janne A. ^{[3
]}

Westenhoff, Sebastian ^{[1
]}

机构：

[1] Univ Gothenburg, Dept Chem & Mol Biol, Box 462, S-40530 Gothenburg, Sweden

[2] Univ Wisconsin, Phys Dept, 3135 North Maryland Ave, Milwaukee, WI 53211 USA

[3] Univ Jyvaskyla, Nanosci Ctr, Dept Biol & Environm Sci, Jyvaskyla 40014, Finland

[4] Univ Helsinki, Fac Med, Dept Anat, Box 63, Helsinki 00014, Finland

[5] Kyoto Univ, Grad Sch Med, Dept Cell Biol, Sakyo Ku, Yoshidakonoe Cho, Kyoto 6068501, Japan

[6] RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan

[7] Japan Synchrotron Radiat Res Inst, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan

[8] Tohoku Univ, Inst Multidisciplinary Res Adv Mat, Aoba Ku, 2-1-1 Katahira, Sendai, Miyagi 9808577, Japan

[9] NE Illinois Univ, Dept Biol, 5500 North St Louis Ave, Chicago, IL 60625 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2021年 / 77卷

基金：

欧洲研究理事会; 芬兰科学院; 美国国家科学基金会;

关键词：

photolyases; flavoproteins; FAD; serial crystallography; room-temperature structure; Drosophila melanogaster; (6-4) photolyase; SERIAL FEMTOSECOND CRYSTALLOGRAPHY; COLI DNA PHOTOLYASE; X-RAY-DIFFRACTION; CRYSTAL-STRUCTURE; RADICAL TRANSFER; REPAIR ACTIVITY; MECHANISM; CRYPTOCHROME; PHOTOACTIVATION; RECONSTITUTION;

D O I：

10.1107/S2059798321005830

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

(6-4) photolyases are flavoproteins that belong to the photolyase/cryptochrome family. Their function is to repair DNA lesions using visible light. Here, crystal structures of Drosophila melanogaster (6-4) photolyase [Dm(6-4)photolyase] at room and cryogenic temperatures are reported. The room-temperature structure was solved to 2.27 angstrom resolution and was obtained by serial femtosecond crystallography (SFX) using an X-ray free-electron laser. The crystallization and preparation conditions are also reported. The cryogenic structure was solved to 1.79 angstrom resolution using conventional X-ray crystallography. The structures agree with each other, indicating that the structural information obtained from crystallography at cryogenic temperature also applies at room temperature. Furthermore, UV-Vis absorption spectroscopy confirms that Dm(6-4)photolyase is photoactive in the crystals, giving a green light to time-resolved SFX studies on the protein, which can reveal the structural mechanism of the photoactivated protein in DNA repair.

引用

页码：1001 / 1009

页数：9

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