Cyclosporine a inhibits the mRNA expressions of IL-2, IL-4 and IFN-γ, but not TNF-α, in canine mononuclear cells

被引：51

作者：

Kobayashi, Tetsuro ^{[1
]}

Momoi, Yasuyuki ^{[1
]}

Iwasaki, Toshiroh ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Fac Agr, Dept Vet Internal Med, Fuchu, Tokyo 1838509, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2007年 / 69卷 / 09期

关键词：

atopic dermatitis; canine; cyclosporine A; cytokines; TNF-alpha;

D O I：

10.1292/jvms.69.887

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The effects of the calcineurin inhibitors cyclosporine A (CsA) and FK506 on the mRNA expressions of various cytokines were evaluated in dogs to determine whether the effects of CsA and FK506 in dogs were similar to those in humans. The mRNA expression levels of the cytokines IL-2, IL-4, IFN-gamma and TNF-alpha were measured in PHA-stimulated canine PBMC using real-time RT-PCR after incubation with CsA or FK506 for 5 hr. Both reagents inhibited IL-2, IL-4 and 1FN-gamma mRNA expressions in a dose-dependent manner. However, CsA hardly inhibited the mRNA expression of TNF-alpha. These findings are important for assessing the indications of CsA treatment in dogs.

引用

页码：887 / 892

页数：6

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