Hydrogen Peroxide Biosensor based on the Immobilization of Horseradish Peroxidase onto a Gold Nanoparticles-Adsorbed Poly(brilliant cresyl blue) Film

被引：21
作者：
Ahammad, A. J. Saleh [1 ]
Shaikh, A. A. [2 ]
Jessy, N. J. [2 ]
Akter, Tania [1 ]
Al Mamun, Abdullah [1 ]
Bakshi, P. K. [2 ]
机构：
[1] Jagannath Univ, Dept Chem, Dhaka 1100, Bangladesh
[2] Univ Dhaka, Dept Chem, Dhaka 1000, Bangladesh
来源：
JOURNAL OF THE ELECTROCHEMICAL SOCIETY | 2015年 / 162卷 / 03期
关键词：
GLASSY-CARBON ELECTRODE; AMPEROMETRIC BIOSENSOR; COLLOIDAL NANOPARTICLES; DIRECT ELECTROCHEMISTRY; POLY(THIONINE) FILM; TOLUIDINE BLUE; SENSOR; HRP; FABRICATION; NANOTUBES;
D O I：
10.1149/2.0211503jes
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
An amperometric hydrogen peroxide (H2O2) biosensor was developed based on the immobilization of horseradish peroxidase (HRP) onto gold nanoparticles (GNPs)-adsorbed conducting poly(brilliant cresyl blue) (PBCB) film. The modification process was characterized by field emission scanning electron microscope (FE-SEM) and electrochemical impedance spectroscopy (EIS). The effects of experimental parameters such as the concentration of the mediator (hydroquinone, HQ), pH of the solution, and the working potential were investigated for optimum analytical performance. In the presence of the mediator, the immobilized HRP showed an excellent electrocatalytic activity towards the reduction of H2O2. The linear dynamic range from 5 to 150 mu M with the regression coefficient of 0.99 was obtained. The detection limit was calculated to be 0.5 mu M based on a signal-to-noise ratio of 3. The reproducibility and stability of the biosensor were studied, with satisfactory results. The biosensor performance was evaluated with respect to possible interferences and the application to real sample analysis. (C) 2014 The Electrochemical Society. All rights reserved.
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收藏
页码：B52 / B56
页数：5
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