Constant serum levels of secreted asialoglycoprotein receptor sH2a and decrease with cirrhosis

被引：9

作者：

Benyair, Ron ^{[1
]}

Kondratyev, Maria ^{[1
]}

Veselkin, Elena ^{[1
]}

Tolchinsky, Sandra ^{[1
]}

Shenkman, Marina ^{[1
]}

Lurie, Yoav ^{[2
]}

Lederkremer, Gerardo Z. ^{[1
]}

机构：

[1] Tel Aviv Univ, Dept Cell Res & Immunol, George Wise Fac Life Sci, IL-69978 Tel Aviv, Israel

[2] Tel Aviv Sourasky Med Ctr, Dept Gastroenterol, Liver Unit, IL-69978 Tel Aviv, Israel

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2011年 / 17卷 / 48期

基金：

以色列科学基金会;

关键词：

Asialoglycoprotein receptor; Hepatitis C virus; Liver function; Cirrhosis; Liver diagnosis; ENDOPLASMIC-RETICULUM; LIVER FIBROSIS; EXPRESSION; AUTOANTIBODIES; MECHANISMS; SUBUNIT;

D O I：

10.3748/wjg.v17.i48.5305

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To investigate the existence and levels of sH2a, a soluble secreted form of the asialoglycoprotein receptor in human serum. METHODS: Production of recombinant sH2a and development of a monoclonal antibody and an enzyme-linked immunosorbent assay (ELISA). This assay was used to determine the presence and concentration of sH2a in human sera of individuals of both sexes and a wide range of ages. RESULTS: The recombinant protein was produced successfully and a specific ELISA assay was developed. The levels of sH2a in sera from 62 healthy individuals varied minimally (147 +/- 19 ng/mL). In contrast, 5 hepatitis C patients with cirrhosis showed much decreased sH2a levels (50 +/- 9 ng/mL). CONCLUSION: Constant sH2a levels suggest constitutive secretion from hepatocytes in healthy individuals. This constant level and the decrease with cirrhosis suggest a diagnostic potential. (C) 2011 Baishideng. All rights reserved.

引用

页码：5305 / 5309

页数：5

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