DNA microarray analysis of white adipose tissue from obese (fa/fa) Zucker rats treated with a β3-adrenoceptor agonist, KTO-7924

被引：12
作者：
Oana, F [1 ]
Homma, T [1 ]
Takeda, H [1 ]
Matsuzawa, A [1 ]
Akahane, S [1 ]
Isaji, M [1 ]
Akahane, M [1 ]
机构：
[1] Kissei Pharmaceut Co Ltd, Div Discovery Res, Nagano 3998304, Japan
来源：
PHARMACOLOGICAL RESEARCH | 2005年 / 52卷 / 05期
关键词：
microarray; beta 3-adrenergic receptor; obesity; lipid oxidation; rat;
D O I：
10.1016/j.phrs.2005.05.014
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
We aimed to examine the effects of KTO-7924 (beta 3-adrenoceptor agonist) on lipid metabolism and mRNA expressions in retroperitoneal white adipose tissue (RP WAT) in obese (fa/fa) Zucker rats using DNA microarray. Oral KTO-7924 for 28 days significantly decreased RP WAT weight, plasma triglyceride, free fatty acid, and insulin, and improved insulin resistance in oral glucose tolerance tests. In RP WAT of KTO-7924-treated rats, DNA microarray analysis revealed specifically enhanced mRNA expressions of uncoupling protein 1 (UCP1) and cytochrome c oxidase subunit VIII-H (COX8H), which are reportedly highly expressed in brown adipose tissue (BAT). Since these mRNA expression levels in RP WAT were significantly lower in obese (fa/fa) Zucker rats than in lean Zucker rats, these genes may be important in lipid metabolism. Our results imply that in obese (fa/fa) Zucker rats, continuous stimulation of beta(3)-adrenoceptors by KTO-7924 causes BAT-like adipocytes to appear in RP WAT, and improves lipid metabolism. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码：395 / 400
页数：6
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