DNA microarray analysis of white adipose tissue from obese (fa/fa) Zucker rats treated with a β3-adrenoceptor agonist, KTO-7924

被引：12

作者：

Oana, F ^{[1
]}

Homma, T ^{[1
]}

Takeda, H ^{[1
]}

Matsuzawa, A ^{[1
]}

Akahane, S ^{[1
]}

Isaji, M ^{[1
]}

Akahane, M ^{[1
]}

机构：

[1] Kissei Pharmaceut Co Ltd, Div Discovery Res, Nagano 3998304, Japan

来源：

PHARMACOLOGICAL RESEARCH | 2005年 / 52卷 / 05期

关键词：

microarray; beta 3-adrenergic receptor; obesity; lipid oxidation; rat;

D O I：

10.1016/j.phrs.2005.05.014

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We aimed to examine the effects of KTO-7924 (beta 3-adrenoceptor agonist) on lipid metabolism and mRNA expressions in retroperitoneal white adipose tissue (RP WAT) in obese (fa/fa) Zucker rats using DNA microarray. Oral KTO-7924 for 28 days significantly decreased RP WAT weight, plasma triglyceride, free fatty acid, and insulin, and improved insulin resistance in oral glucose tolerance tests. In RP WAT of KTO-7924-treated rats, DNA microarray analysis revealed specifically enhanced mRNA expressions of uncoupling protein 1 (UCP1) and cytochrome c oxidase subunit VIII-H (COX8H), which are reportedly highly expressed in brown adipose tissue (BAT). Since these mRNA expression levels in RP WAT were significantly lower in obese (fa/fa) Zucker rats than in lean Zucker rats, these genes may be important in lipid metabolism. Our results imply that in obese (fa/fa) Zucker rats, continuous stimulation of beta(3)-adrenoceptors by KTO-7924 causes BAT-like adipocytes to appear in RP WAT, and improves lipid metabolism. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：395 / 400

页数：6

共 27 条

[1] Leptin signaling [J].

Ahima, RS ;

Osei, SY .

PHYSIOLOGY & BEHAVIOR, 2004, 81 (02) :223-241

[2]

Akahane S, 2004, DIABETOLOGIA, V47, pA256

[3] β3-adrenoceptor agonists:: potential, pitfalls and progress [J].

Arch, JRS .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2002, 440 (2-3) :99-107

[4] Brown adipose tissue: Function and physiological significance [J].

Cannon, B ;

Nedergaard, J .

PHYSIOLOGICAL REVIEWS, 2004, 84 (01) :277-359

[5] Phenotypes of mouse diabetes and rat fatty due to mutations in the OB (leptin) receptor [J].

Chua, SC ;

Chung, WK ;

WuPeng, XS ;

Zhang, YY ;

Liu, SM ;

Tartaglia, L ;

Leibel, RL .

SCIENCE, 1996, 271 (5251) :994-996

[6]

Chung K Fan, 2004, Treat Respir Med, V3, P279, DOI 10.2165/00151829-200403050-00002

[7] GENETIC-VARIATION IN THE BETA(3)-ADRENERGIC RECEPTOR AND AN INCREASED CAPACITY TO GAIN WEIGHT IN PATIENTS WITH MORBID-OBESITY [J].

CLEMENT, K ;

VAISSE, C ;

MANNING, BS ;

BASDEVANT, A ;

GUYGRAND, B ;

RUIZ, J ;

SILVER, KD ;

SHULDINER, AR ;

FROGUEL, P ;

STROSBERG, AD .

NEW ENGLAND JOURNAL OF MEDICINE, 1995, 333 (06) :352-354

[8]

COUSIN B, 1992, J CELL SCI, V103, P931

[9] Beta3-adrenoceptor agonists as anti-diabetic and anti-obesity drugs in humans [J].

de Souza, CJ ;

Burkey, BF .

CURRENT PHARMACEUTICAL DESIGN, 2001, 7 (14) :1433-1449

[10] The adipocyte:: a model for integration of endocrine and metabolic signaling in energy metabolism regulation [J].

Frühbeck, G ;

Gómez-Ambrosi, J ;

Muruzábal, FJ ;

Burrell, MA .

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2001, 280 (06) :E827-E847

← 1 2 3 →