The effects of maternal irradiation during adulthood on mutation induction and transgenerational instability in mice

被引：20

作者：

Ali, Hamdy E. Abouzeid ^{[1
,2
]}

Barber, Ruth C. ^{[1
]}

Dubrova, Yuri E. ^{[1
]}

机构：

[1] Univ Leicester, Dept Genet, Leicester LE1 7RH, Leics, England

[2] Atom Energy Author, Nucl Res Ctr, Radiobiol Applicat Dept, Cairo, Egypt

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 2012年 / 732卷 / 1-2期

关键词：

Radiation; Mutation; Instability; Germline; Mouse; GERM-LINE; GENOMIC INSTABILITY; REPEAT DNA; RADIATION; SPERMATOZOA; MECHANISMS; STABILITY; LOCUS; STAGE; BORN;

D O I：

10.1016/j.mrfmmm.2012.01.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The long-term genetic effects of maternal irradiation remain poorly understood. To establish the effects of radiation exposure on mutation induction in the germline of directly exposed females and the possibility of transgenerational effects in their non-exposed offspring, adult female BALB/c and CBA/Ca mice were given 1 Gy of acute X-rays and mated with control males. The frequency of mutation at expanded simple tandem repeat (ESTR) loci in the germline of directly exposed females did not differ from that of controls. Using a single-molecule PCR approach, ESTR mutation frequency was also established for both germline and somatic tissues in the first-generation offspring of irradiated parents. While the frequency of ESTR mutation in the offspring of irradiated males was significantly elevated, maternal irradiation did not affect stability in their F-1 offspring. Considering these data and the results of our previous study, we propose that, in sharp contrast to paternal exposure to ionising radiation, the transgenerational effects of maternal high-dose acute irradiation are likely to be negligible. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：21 / 25

页数：5

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