Fragile X Related Protein 1 Clusters with Ribosomes and Messenger RNAs at a Subset of Dendritic Spines in the Mouse Hippocampus

被引:37
作者
Cook, Denise [1 ]
Sanchez-Carbente, Maria del Rayo [2 ]
Lachance, Claude [3 ]
Radzioch, Danuta [3 ]
Tremblay, Sandra [4 ]
Khandjian, Edouard W. [4 ]
DesGroseillers, Luc [2 ]
Murai, Keith K. [1 ]
机构
[1] McGill Univ, Ctr Res Neurosci, Montreal Gen Hosp, Dept Neurol & Neurosurg,Res Inst,Hlth Ctr, Montreal, PQ H3A 2T5, Canada
[2] Univ Montreal, Dept Biochim, Montreal, PQ H3C 3J7, Canada
[3] McGill Univ, Div Expt Med, Dept Med, Montreal, PQ H3A 2T5, Canada
[4] Univ Laval, Ctr Rech Robert Giffard, Quebec City, PQ, Canada
基金
加拿大健康研究院; 加拿大自然科学与工程研究理事会; 美国国家卫生研究院;
关键词
MENTAL-RETARDATION PROTEIN; ACTIVITY-DEPENDENT TRAFFICKING; SYNAPTIC PLASTICITY; BINDING PROTEINS; FMRP; GRANULES; FXR1P; LOCALIZATION; TRANSLATION; IDENTIFICATION;
D O I
10.1371/journal.pone.0026120
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The formation and storage of memories in neuronal networks relies on new protein synthesis, which can occur locally at synapses using translational machinery present in dendrites and at spines. These new proteins support long-lasting changes in synapse strength and size in response to high levels of synaptic activity. To ensure that proteins are made at the appropriate time and location to enable these synaptic changes, messenger RNA (mRNA) translation is tightly controlled by dendritic RNA-binding proteins. Fragile X Related Protein 1 (FXR1P) is an RNA-binding protein with high homology to Fragile X Mental Retardation Protein (FMRP) and is known to repress and activate mRNA translation in non-neuronal cells. However, unlike FMRP, very little is known about the role of FXR1P in the central nervous system. To understand if FXR1P is positioned to regulate local mRNA translation in dendrites and at synapses, we investigated the expression and targeting of FXR1P in developing hippocampal neurons in vivo and in vitro. We found that FXR1P was highly expressed during hippocampal development and co-localized with ribosomes and mRNAs in the dendrite and at a subset of spines in mouse hippocampal neurons. Our data indicate that FXR1P is properly positioned to control local protein synthesis in the dendrite and at synapses in the central nervous system.
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页数:17
相关论文
共 68 条
[1]   Dynamic visualization of local protein synthesis in hippocampal neurons [J].
Aakalu, G ;
Smith, WB ;
Nguyen, N ;
Jiang, CG ;
Schuman, EM .
NEURON, 2001, 30 (02) :489-502
[2]  
[Anonymous], 2011, R LANG ENV STAT COMP
[3]   Localization of FMRP-associated mRNA granules and requirement of microtubules for activity-dependent trafficking in hippocampal neurons [J].
Antar, LN ;
Dictenberg, JB ;
Plociniak, M ;
Afroz, R ;
Basselll, GJ .
GENES BRAIN AND BEHAVIOR, 2005, 4 (06) :350-359
[4]   Immunocytochemical and biochemical characterization of FMRP, FXR1P, and FXR2P in the mouse [J].
Bakker, CE ;
Otero, YD ;
Bontekoe, C ;
Raghoe, P ;
Luteijn, T ;
Hoogeveen, AT ;
Oostra, BA ;
Willemsen, R .
EXPERIMENTAL CELL RESEARCH, 2000, 258 (01) :162-170
[5]  
Bardoni Barbara, 2006, Expert Reviews in Molecular Medicine, V8, P1, DOI 10.1017/S1462399406010751
[6]  
Bassell GJ, 1998, J NEUROSCI, V18, P251
[7]   Microarray identification of FMRP-associated brain mRNAs and altered mRNA translational profiles in fragile X syndrome [J].
Brown, V ;
Jin, P ;
Ceman, S ;
Darnell, JC ;
O'Donnell, WT ;
Tenenbaum, SA ;
Jin, XK ;
Feng, Y ;
Wilkinson, KD ;
Keene, JD ;
Darnell, RB ;
Warren, ST .
CELL, 2001, 107 (04) :477-487
[8]   FMRP Stalls Ribosomal Translocation on mRNAs Linked to Synaptic Function and Autism [J].
Darnell, Jennifer C. ;
Van Driesche, Sarah J. ;
Zhang, Chaolin ;
Hung, Ka Ying Sharon ;
Mele, Aldo ;
Fraser, Claire E. ;
Stone, Elizabeth F. ;
Chen, Cynthia ;
Fak, John J. ;
Chi, Sung Wook ;
Licatalosi, Donny D. ;
Richter, Joel D. ;
Darnell, Robert B. .
CELL, 2011, 146 (02) :247-261
[9]   Discrimination of common and unique RNA-binding activities among Fragile X mental retardation protein paralogs [J].
Darnell, Jennifer C. ;
Fraser, Claire E. ;
Mostovetsky, Olga ;
Darnell, Robert B. .
HUMAN MOLECULAR GENETICS, 2009, 18 (17) :3164-3177
[10]   SELECTIVE DENDRITIC TRANSPORT OF RNA IN HIPPOCAMPAL-NEURONS IN CULTURE [J].
DAVIS, L ;
BANKER, GA ;
STEWARD, O .
NATURE, 1987, 330 (6147) :477-479