The cis configuration of amino-modified tetraphenylethene for selective detection of single-stranded guanine-rich DNA

被引：2

作者：

Xu, Li ^{[1
]}

Chen, Zhuo ^{[1
]}

Zhang, Fangdan ^{[1
]}

Luo, Zihan ^{[1
]}

Zheng, Sijia ^{[1
]}

Zhu, Zece ^{[2
]}

Huang, Bing ^{[3
]}

机构：

[1] Hubei Univ Chinese Med, Dept Pharm, Wuhan 430200, Peoples R China

[2] Wuhan Text Univ, Dept Chem & Chem Engn, State Key Lab New Text Mat & Adv Proc Technol, Wuhan 4320200, Peoples R China

[3] Univ Vienna, Fac Phys, A-1090 Vienna, Austria

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2022年 / 272卷

基金：

中国国家自然科学基金;

关键词：

AIE; Configuration; ssDNA; Guanine-rich; Theoretical simulation calculation; MOLECULAR LIGHT SWITCH; FLUORESCENT ASSAY; ETHIDIUM-BROMIDE; AQUEOUS-SOLUTION; NUCLEIC-ACIDS; DERIVATIVES; BINDING; PROBE; HG2+;

D O I：

10.1016/j.saa.2022.120987

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The selectivity and sensitivity of amino-functionalized tetraphenylethene probes (namely Z-N2TPE and E-N2TPE) for ssDNA detection in aqueous solution were investigated. Both Z-N2TPE and E-N2TPE showed high selectivity to guanine-rich ssDNA. The sensitivity was found to be positively related to the DNA length, indicating the longer DNA could binding more probes to cause aggregation induced fluorescence. Z-N2TPE and E-N2TPE could detect guanine-rich ssDNA as short as 5 nt and 10 nt respectively. Theoretical simulation calculation shows that the amino group of the probe could simultaneously bind with guanine and phosphate ester, which contribute to high selectivity. And the cis probe could bind DNA with higher affinity than the trans one, since the two amino groups of Z-N2TPE could synergistically bind DNA while E-N2TPE could bind DNA with only one amino group.(c) 2022 Elsevier B.V. All rights reserved.

引用

页数：6