Guidelines for the Isolation and Characterization of Murine Vascular Smooth Muscle Cells. A Report from the International Society of Cardiovascular Translational Research

被引：34

作者：

Adhikari, Neeta ^{[1
]}

Shekar, Kadambari Chandra ^{[1
]}

Staggs, Rodney ^{[1
]}

Win, Zaw ^{[2
]}

Steucke, Kerianne ^{[2
]}

Lin, Yi-Wei ^{[3
]}

Wei, Li-Na ^{[3
]}

Alford, Patrick ^{[2
]}

Hall, Jennifer L. ^{[1
]}

机构：

[1] Univ Minnesota, Div Cardiol, Lillehei Heart Inst, Dept Med, Minneapolis, MN 55455 USA

[2] Univ Minnesota, Dept Bioengn, Minneapolis, MN 55455 USA

[3] Univ Minnesota, Dept Pharmacol, Minneapolis, MN 55455 USA

来源：

JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH | 2015年 / 8卷 / 03期

关键词：

Vascular smooth muscle cell; Murine; Aorta; Subculture; Caldesmon1; Calponin1; Smooth muscle 22 alpha; Smooth muscle alpha-actin; Smooth muscle myosin heavy chain; Smoothelin; TRACTION FORCE; GROWTH-FACTOR; CULTURE; CONTRACTION; MODULATION; SEROTONIN; MITOGEN; PATHWAY; ADULT;

D O I：

10.1007/s12265-015-9616-6

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Vascular smooth muscle cells (VSMCs) play important roles in cardiovascular disorders and biology. Outlined in this paper is a step-by-step procedure for isolating aortic VSMCs from adult C57BL6J male mice by enzymatic digestion of the aorta using collagenase. The plating, culturing, and subculturing of the isolated cells are discussed in detail along with techniques to characterize VSMC phenotype by gene expression and immunofluorescence. Traction force microscopy was used to characterize contractility of single subcultured VSMCs at baseline.

引用

页码：158 / 163

页数：6

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