A new immobilization system for Candida rugosa lipase: Characterization and applications

A commercial crude lipase from Candida rugosa was immobilized onto special hydrophobic polymers synthetized by us (PVA-CL-Cn) and diatomaceus earth (Celite 545) using buffer (20 mM Hepes and 2 mM EDTA, pH 7.2) as reaction medium. PVA-CL-Cn are polymers made of fatty acids with different carbon chain length, ranging from 4 to 14 carbon atoms. Preoviously, they were employed as column-stationary phases for lipase purification and iso-forms separations. Enzyme preparations were tested in hydrolysis of different tryglicerides. The influence of temperature and chemical nature of supports on enzyme activity and stability were investigated. Results have shown not only a good stability of the biocatalysts (versus time and temperature) but also the efficency of PVA-CL-Cn polymers as lipase immobilization supports (better than Cerite 545).