Detection of anthrax vaccine virulence factors by polymerase chain reaction

被引：57
作者：
Fasanella, A
Losito, S
Trotta, T
Adone, R
Massa, S
Ciuchini, F
Chiocco, D
机构：
[1] Ist Zooprofilatt Sperimentale Puglia & Basilicata, I-71100 Foggia, Italy
[2] Ist Super Sanita, Lab Med Vet, I-00161 Rome, Italy
[3] Univ Foggia, Fac Agr, Ist Prod & Tecnol Alimentari, Foggia, Italy
来源：
VACCINE | 2001年 / 19卷 / 30期
关键词：
anthrax; PCR; vaccine;
D O I：
10.1016/S0264-410X(01)00159-1
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
In Italy, an attenuated Bacillus anthracis strain, named `Carbosap', is used for immunization against ovine and bovine anthrax. Analysis on `Carbosap', Sterne vaccine strain F34 and Pasteur vaccine strain SS104, were performed using primers specific for the sequences, encoding the toxic factors, located on plasmids pXO1 and pXO2 and primers specific for the chromosome. The results obtained from polymerase chain reaction (PCR) assay revealed the presence of both plasmids pXO1 and pXO2 in `Carbosap' strain. This study showed that the `Carbosap' vaccine strain has a different plasmid pattern in comparison to Pasteur vaccine strain SS104 and Sterne vaccine strain F34. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：4214 / 4218
页数：5
相关论文
共 14 条
[1]   NUCLEOTIDE-SEQUENCE AND ANALYSIS OF THE LETHAL FACTOR GENE (LEF) FROM BACILLUS-ANTHRACIS [J].
BRAGG, TS ;
ROBERTSON, DL .
GENE, 1989, 81 (01) :45-54
[2]  
CHIOCCO D, 1985, SIS VET, V39, P657
[3]   STRUCTURAL HOMOLOGY BETWEEN VIRULENCE-ASSOCIATED BACTERIAL ADENYLATE CYCLASES [J].
ESCUYER, V ;
DUFLOT, E ;
SEZER, O ;
DANCHIN, A ;
MOCK, M .
GENE, 1988, 71 (02) :293-298
[4]   IMMUNIZATION STUDIES WITH ATTENUATED STRAINS OF BACILLUS-ANTHRACIS [J].
IVINS, BE ;
EZZELL, JW ;
JEMSKI, J ;
HEDLUND, KW ;
RISTROPH, JD ;
LEPPLA, SH .
INFECTION AND IMMUNITY, 1986, 52 (02) :454-458
[5]   MOLECULAR CHARACTERIZATION AND PROTEIN-ANALYSIS OF THE CAP REGION, WHICH IS ESSENTIAL FOR ENCAPSULATION IN BACILLUS-ANTHRACIS [J].
MAKINO, S ;
UCHIDA, I ;
TERAKADO, N ;
SASAKAWA, C ;
YOSHIKAWA, M .
JOURNAL OF BACTERIOLOGY, 1989, 171 (02) :722-730
[6]   CLONING AND EXPRESSION OF THE CALMODULIN-SENSITIVE BACILLUS-ANTHRACIS ADENYLATE-CYCLASE IN ESCHERICHIA-COLI [J].
MOCK, M ;
LABRUYERE, E ;
GLASER, P ;
DANCHIN, A ;
ULLMANN, A .
GENE, 1988, 64 (02) :277-284
[7]   Molecular characterization of Bacillus strains involved in outbreaks of anthrax in France in 1997 [J].
Patra, G ;
Vaissaire, J ;
Weber-Levy, M ;
Le Doujet, C ;
Mock, M .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (11) :3412-3414
[8]  
PATRA G, 1996, SALISBURY MED B S, V87, P45
[9]   Identification and characterization of Bacillus anthracis by multiplex PCR analysis of sequences on plasmids pXO1 and pXO2 and chromosomal DNA [J].
Ramisse, V ;
Patra, G ;
Garrigue, H ;
Guesdon, JL ;
Mock, M .
FEMS MICROBIOLOGY LETTERS, 1996, 145 (01) :9-16
[10]  
ROBERTSON DL, 1989, SALISBURY MED B, P55
12