The path of messenger RNA through the ribosome

被引：478
作者：
Yusupova, GZ
Yusupov, MM
Cate, JHD
Noller, HF [1 ]
机构：
[1] Univ Calif Santa Cruz, Sinsheimer Labs, Ctr Mol Biol RNA, Santa Cruz, CA 95064 USA
[2] Whitehead Inst, Cambridge, MA 01242 USA
来源：
CELL | 2001年 / 106卷 / 02期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1016/S0092-8674(01)00435-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Using X-ray crystallography, we have directly observed the path of mRNA in the 70S ribosome in Fourier difference maps at 7 Angstrom resolution. About 30 nucleotides of the mRNA are wrapped in a groove that encircles the neck of the 30S subunit. The Shine-Dalgarno helix is bound in a large cleft between the head and the back of the platform. At the interface, only about eight nucleotides (-1 to +7), centered on the junction between the A and P codons, are exposed, and bond almost exclusively to 16S rRNA. The mRNA enters the ribosome around position + 13 to + 15, the location of downstream pseudoknots that stimulate -1 translational frame shifting.
引用
收藏
页码：233 / 241
页数：9
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