RNA aptamer based electrochemical biosensor for sensitive and selective detection of cAMP

被引：48

作者：

Zhao, Fulin ^{[1
]}

Xie, Qingyun ^{[1
]}

Xu, Mingfei ^{[1
]}

Wang, Shouyu ^{[2
]}

Zhou, Jiyong ^{[1
]}

Liu, Fei ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China

[2] Nanjing Univ Sci & Technol, Dept Informat Phys & Engn, Nanjing 210094, Jiangsu, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2015年 / 66卷

基金：

中国国家自然科学基金;

关键词：

RNA aptamer; Electrochemical impedance spectroscopy; Gold nanoparticles; Cyclic adenosine monophosphate; HORMONE-RELATED PROTEIN-(1-36); VITAMIN-D DEFICIENCY; LABEL-FREE; GOLD NANOPARTICLE; APTASENSOR; RECOGNITION; ADENOSINE; CELLS; ACTIVATION; EXCRETION;

D O I：

10.1016/j.bios.2014.11.024

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Cyclic adenosine monophosphate (cAMP) is an important small biological molecule associated with the healthy state of living organism. In order to realize highly sensitive and specific detection of cAMP, here an RNA aptamer and electrochemical impedance spectroscopy (EIS) based biosensor enhanced by gold nanoparticles electrodeposited on the surface of gold electrode is designed. The designed aptasensor has a wide effective measuring range from 50 pM to 250 pM with a detection limit of 50 pM in PBS buffer, and an effective measuring range from 50 nM to 1 mu M with a detection limit of 50 nM in serum. The designed biosensor is also able to detect cAMP with high sensitivity, specificity, and stability. Since the biosensor can be easily fabricated with low cost and repeatedly used for at least two times, it owns great potential in wide application fields such as clinical test and food inspection, etc. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：238 / 243

页数：6

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