Sparking new frontiers:: Using in vivo electroporation for genetic manipulations

被引：127

作者：

Swartz, M

Eberhart, J

Mastick, GS

Krull, CE

机构：

[1] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA

[2] Univ Nevada, Dept Biol, Reno, NV 89557 USA

[3] Univ Nevada, Dept Biochem, Reno, NV 89557 USA

来源：

DEVELOPMENTAL BIOLOGY | 2001年 / 233卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1006/dbio.2001.0181

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In vivo electroporation is a fascinating new approach by which gene expression, regulation, and function can be studied in developmental systems. This technique offers new opportunities for manipulations in animal models that lack genetic approaches, including avians. Furthermore, this approach is applicable to other embryo populations including mice, ascidians, zebrafish, Xenopus, and Drosophila. In this review, we discuss technical aspects of in vivo electroporation, review recent studies where this approach has been utilized successfully, and identify future directions. (C) 2001 Academic Press.

引用

页码：13 / 21

页数：9

共 57 条

[1] Mammalian ELAV-like neuronal RNA-binding proteins HuB and HuC promote neuronal development in both the central and the peripheral nervous systems [J].