Knockdown of PKR expression by RNAi reduces pulmonary metastatic potential of B16-F10 melanoma cells in mice:: Possible role of NF-κB

被引：19

作者：

Andre, Nayara Delgado ^{[1
]}

De Lucca, Fernando L. ^{[1
]}

机构：

[1] Univ Sao Paulo, Sch Med, Dept Biochem & Immunol, BR-14049900 Ribeirao Preto, Brazil

来源：

CANCER LETTERS | 2007年 / 258卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

PKR; RNA interference; B16; melanoma; metastasis; cancer; NF-kappa B;

D O I：

10.1016/j.canlet.2007.08.021

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

It was suggested that PKR may act as a tumor suppressor. However, the results are still controversial. Here, we investigate the effect of PKR-specific short hairpin RNA (shRNA) expressing plasmids on pulmonary metastatic potential of B16-F10 melanoma cells in mice. Levels of both PKR mRNA and PKR protein were only significantly reduced in tumor cells transfected with plasmid-based PKR-specific shRNA. Injection of tumor cells transfected with PKR-specific shRNA expressing plasmid resulted in inhibition (85%) of metastatic nodules compared with control. Our results suggest that this effect is mediated by the transcription factor NF-kappa B. This study does not support the concept of PKR as a tumor suppressor. (c) 2007 Elsevier Ireland Ltd. All rights reserved.

引用

页码：118 / 125

页数：8

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