Evidence for an unfolding and refolding pathway in cytochrome c

被引：136

作者：

Xu, YJ ^{[1
]}

Mayne, L ^{[1
]}

Englander, SW ^{[1
]}

机构：

[1] Univ Penn, Dept Biochem & Biophys, Johnson Res Fdn, Philadelphia, PA 19104 USA

来源：

NATURE STRUCTURAL BIOLOGY | 1998年 / 5卷 / 09期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/1810

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has been suggested that three partially unfolded forms detected in a native state hydrogen exchange study of oxidized cytochrome c may represent sequential intermediates in an unfolding-refolding pathway. To better define the structure of each intermediate a 'stability labeling' method was used in which the stability of a given segment against unfolding was changed. The condition - folded or unfolded - of the stability-labeled segment in each intermediate could then be determined. The structures found are discrete and native-like and are just those necessary for a sequential pathway.

引用

页码：774 / 778

页数：5

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