Grb2 promotes reinitiation of meiosis in Xenopus oocytes

被引：13
作者：
Cailliau, K
Browaeys-Poly, E
Broutin-L'Hermite, I
Nioche, P
Garbay, C
Ducruix, A
Vilain, JP
机构：
[1] Univ Sci & Technol Lille, UE 1033, Lab Biol Dev, F-59655 Villeneuve Dascq, France
[2] Univ Paris 05, Fac Pharm, CNRS EP 2075, Lab Cristallog & RMN Biol, F-75270 Paris 06, France
[3] Univ Paris 05, Fac Pharm, CNRS UMR 8600, INSERM U266,Unite Pharmacochim Mol & Struct, F-75270 Paris 06, France
来源：
CELLULAR SIGNALLING | 2001年 / 13卷 / 01期
关键词：
Grb2; meiotic maturation; MAP kinase; Xenopus laevis oocyte;
D O I：
10.1016/S0898-6568(00)00138-8
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The adaptor protein Grb2 plays a central role in cell proliferation and/or cell cycle progression. In this study, we investigate the role of Grb2 in signalling pathways involved in meiotic reinitiation. For that purpose, Xenopus Grb2 cRNA and its mutated forms or human Grb2 protein was microinjected into immature Xenopus oocytes. Reinitiation of meiosis was seen in unstimulated oocytes. Induction of the meiosis was time dependent and Ras dependent, and the presence in Grb2 of SH2 and SH3 domains was required. Several tyrosine phosphorylated proteins were solely detected in oocytes responsive to Grb2 injection. Our results are in favour of an unusual recruitment and initiation of the Grb2 transduction cascade independent of a receptor tyrosine kinase (RTK) stimulation. (C) 2001 Elsevier Science Inc. All rights reserved.
引用
收藏
页码：51 / 55
页数：5
相关论文
共 26 条
[1]  
Aroca P, 1996, ONCOGENE, V13, P1839
[2]  
AZZI L, 1994, J BIOL CHEM, V269, P13279
[3]   EARLY INCREASE OF A 105,000-DALTON PHOSPHOPROTEIN DURING MEIOTIC MATURATION OF XENOPUS-LAEVIS OOCYTE [J].
BOYER, J ;
BELLE, R ;
CAPONY, JP ;
OZON, R .
BIOCHIMIE, 1983, 65 (01) :15-23
[4]   Fibroblast and epidermal growth factor receptor expression in Xenopus oocytes displays distinct calcium oscillatory patterns [J].
Browaeys-Poly, E ;
Cailliau, K ;
Vilain, JP .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1998, 1404 (03) :484-489
[5]   THE GRB2 ADAPTER [J].
CHARDIN, P ;
CUSSAC, D ;
MAIGNAN, SB ;
DUCRUIX, A .
FEBS LETTERS, 1995, 369 (01) :47-51
[6]   INSULIN-STIMULATED OOCYTE MATURATION REQUIRES INSULIN-RECEPTOR SUBSTRATE-1 AND INTERACTION WITH THE SH2 DOMAINS OF PHOSPHATIDYLINOSITOL 3-KINASE [J].
CHUANG, LM ;
MYERS, MG ;
BACKER, JM ;
SHOELSON, SE ;
WHITE, MF ;
BIRNBAUM, MJ ;
KAHN, CR .
MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (11) :6653-6660
[7]   C-ELEGANS CELL-SIGNALING GENE SEM-5 ENCODES A PROTEIN WITH SH2 AND SH3 DOMAINS [J].
CLARK, SG ;
STERN, MJ ;
HORVITZ, HR .
NATURE, 1992, 356 (6367) :340-344
[8]   INSULIN INDUCTION OF XENOPUS-LAEVIS OOCYTE MATURATION IS INHIBITED BY MONOCLONAL-ANTIBODY AGAINST P21 RAS PROTEINS [J].
DESHPANDE, AK ;
KUNG, HF .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (03) :1285-1288
[9]   THE GRB2/SEM-5 ADAPTER PROTEIN [J].
DOWNWARD, J .
FEBS LETTERS, 1994, 338 (02) :113-117
[10]   The biochemical basis of an all-or-none cell fate switch in Xenopus oocytes [J].
Ferrell, JE ;
Machleder, EM .
SCIENCE, 1998, 280 (5365) :895-898
123