Effects of three esca-associated fungi on Vitis vinifera L.:: I.: Characterization of secondary metabolites in culture media and host responses to the pathogens in calli

被引：51

作者：

Bruno, Giovanni ^{[1
]}

Sparapano, Lorenzo ^{[1
]}

机构：

[1] Univ Bari, Dipartmento Biol & Paotl Vegtale, I-70126 Bari, Italy

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 2006年 / 69卷 / 4-6期

关键词：

Vitis vinifera; Phaeomoniella chlamydospora; Togninia minima; Fomitiporia mediterranea; esca syndrome; plant-pathogen interactions; calli; phenolics; phytoalexins; pentaketides; pullulans;

D O I：

10.1016/j.pmpp.2007.04.008

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Phaeomoniella chlamydospora (Pch) and Togninia minima (Tmi) produced scytalone, isosclerone and pullulans in liquid cultures, as well as in calli. Secondary metabolites and host defense Compounds were shown to occur in calli of Vitis vinfera cv. Italia and cv. Matilde infected by the esca-associated fungi Pch, Tmi and Fomitiporia mediterranea (Fme). Calli of both cvs. were grown as dual cultures with Pch. Tmi and Fme. The fungi grew well in the presence of calli of both cvs., but callus growth was reduced. Accumulation and changes of total phenolics and recurring phenolics, and of two phytotoxic pentaketides and pullulans were analyzed by HPLC. On comparing results for cv. Italia and cv. Matilde, it can be seen that concentrations of phenolics are strongly related to the cv. The paper discusses the possible relationship between melanin biosynthesis in Pch and Did, which utilize pentaketide metabolites as intermediates and their pathogenicity related to phytotoxity of scytalone and isosclerone. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：209 / 223

页数：15

共 58 条

[1]

BARKER S A, 1956, Methods Biochem Anal, V3, P213, DOI 10.1002/9780470110195.ch7

[2] Characterization of a pterostilbene dehydrodimer produced by laccase of Botrytis cinerea [J].