Study on the binding equilibrium between surfactant FC95 and DNA by resonance light-scattering technique

被引：14

作者：

Li, Ling ^{[1
]}

Pan, Quan ^{[1
]}

Wang, Ying Xi ^{[1
]}

Song, Gong Wu ^{[1
]}

Xu, Zu Shun ^{[1
]}

机构：

[1] Hubei Univ, Minist Educ, Key Lab Synth & Applicat Organ Funct Mol, Wuhan 430062, Peoples R China

来源：

APPLIED SURFACE SCIENCE | 2011年 / 257卷 / 10期

关键词：

Fluorinated surfactant; DNA; Resonance light scattering; Conformation; GEMINI SURFACTANTS; NUCLEIC-ACIDS;

D O I：

10.1016/j.apsusc.2010.10.024

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The binding equilibrium of potassium perfluorooctanesulfonate (FC95) to DNA was studied by UV spectra and resonance light scattering technique in the present paper. It was found that the interaction resulted in strongly enhanced resonance light scattering signals characterized by the RLS peak at 370 nm. The results indicated that the conformation of the DNA has changed during the interaction because the micro environment of DNA changed. The binding constant K-f and the number of binding n were calculated according to the equilibrium equation, which was established based on the data of the resonance light scattering measurements at 370 nm. It was found that the binding K-f and the number of binding n were 6.02x10(3) and 2.0, respectively. (C) 2010 Elsevier B. V. All rights reserved.

引用

页码：4547 / 4551

页数：5

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