Generation of synchronized picosecond pulses by a 1.06-μm gain-switched laser diode for stimulated Raman scattering microscopy

被引：12

作者：

Tokunaga, Kyoya ^{[1
]}

Fang, Yi-Cheng ^{[2
]}

Yokoyama, Hiroyuki ^{[2
]}

Ozeki, Yasuyuki ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Engn, Dept Elect Engn & Informat Syst, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138656, Japan

[2] Tohoku Univ, New Ind Creat Hatchery Ctr NICHe, Aoba Ku, 6-6-10 Aramaki Aoba, Sendai, Miyagi 9808579, Japan

来源：

OPTICS EXPRESS | 2016年 / 24卷 / 09期

关键词：

IMAGING IN-VIVO; LABEL-FREE; OPTICAL PULSE; SPECTRAL MICROSCOPY; BALANCED DETECTION; LIVE CELLS; SENSITIVITY; CHOLESTEROL; VISUALIZATION; OSCILLATOR;

D O I：

10.1364/OE.24.009617

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We propose that a gain-switched laser diode (GS-LD) can be used as a picosecond laser source for stimulated Raman scattering (SRS) microscopy. We employed a 1.06-mu m GS-LD to generate similar to 13-ps pulses at a repetition rate of 38 MHz and amplified them to > 100 mW with Yb-doped fiber amplifiers. The GS-LD was driven by 200-ps electrical pulses, which were triggered through a toggle flip-flop (T-FF) so that the GS-LD pulses were synchronized to Ti: sapphire laser (TSL) pulses at a repetition rate of 76 MHz. We found the timing jitter of GS-LD pulses to be approximately 2.7 ps in a jitter bandwidth of 7 MHz. We also show that the delay of electrical pulses can be less sensitive to the optical power of TSL pulses by controlling the threshold voltage of the T-FF. We demonstrate the SRS imaging of polymer beads and of HeLa cells with GS-LD pulses and TSL pulses, proving that GS-LD is readily applicable to SRS microscopy as a compact and stable pulse source. (C) 2016 Optical Society of America

引用

页码：9617 / 9628

页数：12

共 59 条

[1] Sub-5-ps optical pulse generation from a 1.55-μm distributed-feedback laser diode with nanosecond electric pulse excitation and spectral filtering [J].