Multi-functional self-fluorescent unimolecular micelles for tumor-targeted drug delivery and bioimaging

被引：95

作者：

Chen, Guojun ^{[1
,2
]}

Wang, Liwei ^{[2
,3
]}

Cordie, Travis ^{[3
]}

Vokoun, Corinne ^{[4
]}

Eliceiri, Kevin W. ^{[4
]}

Gong, Shaoqin ^{[1
,2
,3
,5
]}

机构：

[1] Univ Wisconsin, Mat Sci Program, Madison, WI 53715 USA

[2] Univ Wisconsin, Wisconsin Inst Discovery, Madison, WI 53715 USA

[3] Univ Wisconsin, Dept Biomed Engn, Madison, WI 53715 USA

[4] Univ Wisconsin, Lab Opt & Computat Instrumentat, Madison, WI 53715 USA

[5] Univ Wisconsin, Carbone Canc Ctr, Madison, WI 53715 USA

来源：

BIOMATERIALS | 2015年 / 47卷

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

Self-fluorescence; Unimolecular micelles; Drug nanocarrier; Drug delivery; Bioimaging probe; Cancer theranostics; QUANTUM DOTS; CANCER-THERAPY; INTEGRIN ALPHA(V)BETA(3); NANOPARTICLES; NANOCARRIERS; DOXORUBICIN; PHOTOLUMINESCENT; CYTOTOXICITY; SYSTEMS;

D O I：

10.1016/j.biomaterials.2015.01.006

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A novel type of self-fluorescent unimolecular micelle nanoparticle (NP) formed by multi-arm star amphiphilic block copolymer, Boltron(R) H40 (H40, a 4th generation hyperbranched polymer)-biodegradable photo-luminescent polymer (BPLP)-poly(ethylene glycol) (PEG) conjugated with cRGD peptide (i.e., H40-BPLP-PEG-cRGD) was designed, synthesized, and characterized. The hydrophobic BPLP segment was self-fluorescent, thereby making the unimolecular micelle NP self-fluorescent. cRGD peptides, which can effectively target alpha(v)beta(3) integrin-expressing tumor neovasculature and tumor cells, were selectively conjugated onto the surface of the micelles to offer active tumor-targeting ability. This unique self-fluorescent unimolecular micelle exhibited excellent photostability and low cytotoxicity, making it an attractive bioimaging probe for NP tracking for a variety of microscopy techniques including fluorescent microscopy, confocal laser scanning microscopy (CLSM), and two-photon microscopy. Moreover, this self-fluorescent unimolecular micelle NP also demonstrated excellent stability in aqueous solutions due to its covalent nature, high drug loading level, pH-controlled drug release, and passive and active tumor-targeting abilities, thereby making it a promising nanoplatform for targeted cancer theranostics. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：41 / 50

页数：10

共 42 条

[21] Amphiphilic multiarm star block copolymer-based multifunctional unimolecular micelles for cancer targeted drug delivery and MR imaging [J].

Li, Xiaojie ;

Qian, Yinfeng ;

Liu, Tao ;

Hu, Xianglong ;

Zhang, Guoying ;

You, Yezi ;

Liu, Shiyong .

BIOMATERIALS, 2011, 32 (27) :6595-6605

[22] cRGD-functionalized polymer micelles for targeted doxorubicin delivery [J].

Nasongkla, N ;

Shuai, X ;

Ai, H ;

Weinberg, BD ;

Pink, J ;

Boothman, DA ;

Gao, JM .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (46) :6323-6327

[23] Polymeric Micelles in Anticancer Therapy: Targeting, Imaging and Triggered Release [J].

Oerlemans, Chris ;

Bult, Wouter ;

Bos, Mariska ;

Storm, Gert ;

Nijsen, J. Frank W. ;

Hennink, Wim E. .

PHARMACEUTICAL RESEARCH, 2010, 27 (12) :2569-2589

[24] Quantitative and reversible lectin-induced association of gold nanoparticles modified with α-lactosyl-ω-mercapto-poly(ethylene glycol) [J].

Otsuka, H ;

Akiyama, Y ;

Nagasaki, Y ;

Kataoka, K .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (34) :8226-8230

[25] Opsonization, biodistribution, and pharmacokinetics of polymeric nanoparticles [J].

Owens, DE ;

Peppas, NA .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2006, 307 (01) :93-102

[26] Cooperative Nanoparticles for Tumor Detection and Photothermally Triggered Drug Delivery [J].

Park, Ji-Ho ;

von Maltzahn, Geoffrey ;

Ong, Luvena L. ;

Centrone, Andrea ;

Hatton, T. Alan ;

Ruoslahti, Erkki ;

Bhatia, Sangeeta N. ;

Sailor, Michael J. .

ADVANCED MATERIALS, 2010, 22 (08) :880-+

[27] Amphiphilic multi-arm-block copolymer conjugated with doxorubicin via pH-sensitive hydrazone bond for tumor-targeted drug delivery [J].

Prabaharan, Mani ;

Grailer, Jamison J. ;

Pilla, Srikanth ;

Steeber, Douglas A. ;

Gong, Shaoqin .

BIOMATERIALS, 2009, 30 (29) :5757-5766

[28] Folate-conjugated amphiphilic hyperbranched block copolymers based on Boltorn® H40, poly(L-lactide) and poly(ethylene glycol) for tumor-targeted drug delivery [J].

Prabaharan, Mani ;

Grailer, Jamison J. ;

Pilla, Srikanth ;

Steeber, Douglas A. ;

Gong, Shaoqin .

BIOMATERIALS, 2009, 30 (16) :3009-3019

[29] Doxorubicin: an update on anticancer molecular action, toxicity and novel drug delivery systems [J].

Tacar, Oktay ;

Sriamornsak, Pornsak ;

Dass, Crispin R. .

JOURNAL OF PHARMACY AND PHARMACOLOGY, 2013, 65 (02) :157-170

[30] Micellar nanocarriers: Pharmaceutical perspectives [J].

Torchilin, V. P. .

PHARMACEUTICAL RESEARCH, 2007, 24 (01) :1-16

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