Identification and immunohistochemical localization of Sulfotransferase 2B1b (SULT2B1b) in human lung

被引：27
作者：
He, DN
Frost, AR
Falany, CN
机构：
[1] Univ Alabama Birmingham, Dept Pharmacol & Toxicol, Birmingham, AL 35249 USA
[2] Univ Alabama Birmingham, Dept Pathol, Birmingham, AL 35249 USA
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2005年 / 1724卷 / 1-2期
基金：
美国国家卫生研究院;
关键词：
sulfotransferase; lung; SULT2B1b; sulfation; immunohistochemical localization;
D O I：
10.1016/j.bbagen.2005.03.018
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Sulfotransferase 2B1b (SULT2B1b) is a member of SULT 2 gene family. SUMB1a and SULT2B1b are transcribed from the same gene using different transcriptional start sites and contain different first exons as the result of alternative splicing. SULT2B1 a and SULT2B1b are 350 and 365 AA in length, respectively. Northern blot analysis and SULT2B1 isoform specific RT-PCR detected only SULT2B1 message in human lung tissue. Immunoblot analysis of human lung tissue with a specific rabbit anti-SUMB1 polyclonal antibody detected only SULT2B1b immunoreactive protein in human lung cytosol. Immunoprecipitation and MALDI mass spectroscopic analysis verified that the immunoreactive protein was SULT2B1b. Immunohistochemical localization of SULT2B1b in human tissues showed expression in the cytoplasm of ciliated columnar or cuboidal epithelial cells in terminal bronchia. No staining was observed in alveolar cells. SULT2B1b is selective for the sulfation of 3 beta-hydroxysteroids such as dehydroepiandrosterone and pregnenolone as well as cholesterol. The presence of SULT2B1b in lung tissues suggests a role in the regulation of local steroid hormone synthesis and metabolism. (c) 2005 Elsevier B.V All rights reserved.
引用
收藏
页码：119 / 126
页数：8
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