The effect of resveratrol on a cell model of human aging

被引：60

作者：

Stefani, Maurizio ^{[1
]}

Markus, M. Andrea ^{[1
]}

Lin, Ruby C. Y. ^{[2
]}

Pinese, Mark ^{[2
]}

Dawes, Ian W. ^{[2
]}

Morris, Brian J. ^{[1
]}

机构：

[1] Univ Sydney, Sch Med Sci, Basic & Clin Genom Lab, Sydney, NSW 2006, Australia

[2] Univ New S Wales, Ramaciotti Ctr Gene Funct Anal, Sydney, NSW, Australia

来源：

HEALTHY AGING AND LONGEVITY | 2007年 / 1114卷

关键词：

resveratrol; nicotinamide; senescence; cell culture; microarray; fibroblasts;

D O I：

10.1196/annals.1396.001

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

The natural polyphenol resveratrol stimulates sirtuins and extends lifespan. Here resveratrol inhibited expression of replicative senescence marker INK4a in human dermal fibroblasts, and 47 of 19,000 genes from microarray experiments were differentially expressed. These included genes for growth, cell division, cell signaling, apoptosis, and transcription. Genes involved in Ras and ubiquitin pathways, Ras-GRF1, RAC3, and UBE2D3, were downregulated. The changes suggest resveratrol might alter sirtuin-regulated downstream pathways, rather than sirtuin activity. Serum deprivation and high confluency caused nuclear translocation of the SIRT1-regulated transcription factor FOXO3a. Our data indicate resveratrol's actions might cause FOXO recruitment to the nucleus.

引用

页码：407 / 418

页数：12

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