Nanofibrous polysaccharide hydroxyapatite composites with biocompatibility against human osteoblasts

被引:20
作者
Gasparic, Petra [1 ]
Kurecic, Manja [1 ,2 ,4 ]
Kargl, Rupert [1 ,4 ]
Maver, Uros [3 ]
Gradisnik, Lidija [3 ]
Hribernik, Silvo [1 ]
Kleinschek, Karin Stana [1 ,4 ]
Smole, Majda Sfiligoj [1 ]
机构
[1] Univ Maribor, Fac Mech Engn, Lab Characterisat & Proc Polymers, Smetanova 17, SI-2000 Maribor, Slovenia
[2] Ctr Excellence PoliMaT, Tehnol Pk 24, SI-1000 Ljubljana, Slovenia
[3] Univ Maribor, Fac Med, Inst Biomed Sci, Taborska Ulica 8, SI-2000 Maribor, Slovenia
[4] Graz Univ Technol, Inst Chem & Technol Mat, Stremayrgasse 9, A-8010 Graz, Austria
关键词
Carboxymethyl cellulose; Nanofibers; Electrospinning; Hydroxyapatite; Cell viability; Osteoblasts; BONE; NANOPARTICLES; BIOMATERIALS; SCAFFOLDS; APATITES; CALCIUM; BIOLOGY; CELLS;
D O I
10.1016/j.carbpol.2017.08.111
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Regenerative medicine has a high demand for defined scaffold materials that promote cell growth, stabilize the tissue during maturation and provide a proper three dimensional structure that allows the exchange of nutrients. In many instances nanofiber composites have already shown their potential for such applications. This work elaborates the development of polysaccharide based nanofibers with integrated hydroxyapatite nanoparticles. A detailed study on the formation of electrospun nanofibres from aqueous mixtures of carboxymethyl cellulose polyethylene oxide was performed. The influence of different processing conditions and spinning solution properties using a nozzle-less electrospinning device was systematically studied. Optimized parameters were used to incorporate hydroxyapatite nanoparticles into the fibers. Nanofibers were additionally hydrophobized with alkenyl succinic anhydride (ASA) to render them insoluble in water. The nanofiber webs were thoroughly investigated with respect to morphology, chemical composition and inorganic content. Time dependent bio-compatibility testing of the materials with human bone-derived osteoblasts showed no significant reduction in cell viability for the developed materials composed of carboxymethyl cellulose/polyethyleneoxide. Cells grown on hydrophobized materials show similar viability as those grown on a commercial collagen/apatite matrix.
引用
收藏
页码:388 / 396
页数:9
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