Detection Of Viable Listeria monocytogenes In Dairy Products By Real Time Reverse-transcription PCR

被引：0
|
作者：
Yan, Bing [1 ]
Zhao, Feng [1 ]
Bi, Yuhan [1 ]
Xiang, Li [1 ]
Jiang, Yujun [1 ]
机构：
[1] NE Agr Univ, Minist Educ, Key Lab Dairy Sci, Harbin, Peoples R China
来源：
2009 3RD INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1-11 | 2009年
关键词：
real time RT-PCR; Listeria monocytogenes; biofilm; BIOFILMS;
D O I：
暂无
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Listeria monocytogenes is a food-borne pathogen which can cause zoonosis. A detection method based on real time reverse-transcription PCR amplification of mRNA was established in the study. This method can overcome false-positive result caused by amplification of nonviable L.monocytogenes. Sensitivity and specificity of the method were studied, as well as artificially contaminated dairy products by L.monocytogenes, dairy products from market and L.monocytogenes biofilm. For contaminated milk, detection sensitivity was 17CFU/ml after 6h enrichment. Detection limit in biofilm was 2x10(2)CFU/cm(2) after 7h enrichment. The results indicate that the method can satisfy detection of L.monocytogenes in dairy products and biofilm.
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收藏
页码：3292 / 3294
页数：3
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