Protocol Protocol for correlation analysis of the murine gut microbiome and meta-metabolome using 16S rDNA sequencing and UPLC-MS

被引：1
|
作者：
Li, Xiaoqing [1 ,2 ,3 ,4 ]
Wu, Peng [7 ]
Zeng, Xiangjun [1 ,2 ,3 ,4 ]
Lang, Qiulei [7 ]
Lin, Yu [1 ,2 ,3 ,4 ]
Huang, He [1 ,2 ,3 ,4 ]
Qian, Pengxu [2 ,3 ,4 ,5 ,6 ]
机构：
[1] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Bone Marrow Transplantat Ctr, Hangzhou, Zhejiang, Peoples R China
[2] Zhejiang Univ, Med Ctr, Liangzhu Lab, Hangzhou, Peoples R China
[3] Zhejiang Univ, Inst Hematol, Hangzhou, Peoples R China
[4] Zhejiang Engn Lab Stem Cell & Immunotherapy, Hangzhou, Zhejiang, Peoples R China
[5] Zhejiang Univ, Sch Med, Ctr Stem Cell & Regenerat Med, Hangzhou 310058, Peoples R China
[6] Zhejiang Univ, Bone Marrow Transplantat Ctr Affiliated Hosp 1, Sch Med, Hangzhou 310058, Peoples R China
[7] LC BioTechnol Co Ltd, Hangzhou, Zhejiang, Peoples R China
来源：
STAR PROTOCOLS | 2022年 / 3卷 / 03期
基金：
国家重点研发计划; 中国国家自然科学基金;
关键词：
SPECTROMETRY;
D O I：
10.1016/j.xpro.2022.101494
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The gut microbiota and metabolites play pivotal roles in the pathobiology of various diseases. Here, we describe a protocol to profile the gut microbiome and meta-metabolome of a mouse disease model for acute graft-versus-host dis-ease. We describe steps for fecal sample collection and processing for 16S sequencing and UPLC-MS. Finally, we detail the steps for data analysis and exhibit multi-omic associations to correlate with pathology. For complete details on the use and execution of this protocol, please refer to Li et al. (2020).
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收藏
页数：20
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