Protocol Protocol for correlation analysis of the murine gut microbiome and meta-metabolome using 16S rDNA sequencing and UPLC-MS

被引：1

作者：

Li, Xiaoqing ^{[1
,2
,3
,4
]}

Wu, Peng ^{[7
]}

Zeng, Xiangjun ^{[1
,2
,3
,4
]}

Lang, Qiulei ^{[7
]}

Lin, Yu ^{[1
,2
,3
,4
]}

Huang, He ^{[1
,2
,3
,4
]}

Qian, Pengxu ^{[2
,3
,4
,5
,6
]}

机构：

[1] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Bone Marrow Transplantat Ctr, Hangzhou, Zhejiang, Peoples R China

[2] Zhejiang Univ, Med Ctr, Liangzhu Lab, Hangzhou, Peoples R China

[3] Zhejiang Univ, Inst Hematol, Hangzhou, Peoples R China

[4] Zhejiang Engn Lab Stem Cell & Immunotherapy, Hangzhou, Zhejiang, Peoples R China

[5] Zhejiang Univ, Sch Med, Ctr Stem Cell & Regenerat Med, Hangzhou 310058, Peoples R China

[6] Zhejiang Univ, Bone Marrow Transplantat Ctr Affiliated Hosp 1, Sch Med, Hangzhou 310058, Peoples R China

[7] LC BioTechnol Co Ltd, Hangzhou, Zhejiang, Peoples R China

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 03期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

SPECTROMETRY;

D O I：

10.1016/j.xpro.2022.101494

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The gut microbiota and metabolites play pivotal roles in the pathobiology of various diseases. Here, we describe a protocol to profile the gut microbiome and meta-metabolome of a mouse disease model for acute graft-versus-host dis-ease. We describe steps for fecal sample collection and processing for 16S sequencing and UPLC-MS. Finally, we detail the steps for data analysis and exhibit multi-omic associations to correlate with pathology. For complete details on the use and execution of this protocol, please refer to Li et al. (2020).

引用

页数：20

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