Uniform Amplification of Phage with Different Growth Characteristics in Individual Compartments Consisting of Monodisperse Droplets

被引：48

作者：

Derda, Ratmir ^{[1
]}

Tang, Sindy K. Y. ^{[1
]}

Whitesides, George M. ^{[1
]}

机构：

[1] Harvard Univ, Wyss Inst Biol Inspired Engn, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 31期

基金：

美国国家科学基金会;

关键词：

competition; microfluidics; monodisperse colloids; phage-display; IN-VITRO SELECTION; PEPTIDE LIBRARIES; FILAMENTOUS PHAGE; SURFACE DISPLAY; DNA DISPLAY; EVOLUTION; PROTEIN; TECHNOLOGIES; FUSION; CELLS;

D O I：

10.1002/anie.201001143

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Save every clonel In phage display, clones displaying ligands that hinder growth of phage are lost in amplification. Competition of slowly (S) and rapidly (R) growing phage is mitigated in monodisperse emulsions generated by a simple microfluidic device. Separating R and S in ca. 107 droplets maintains R/S ratio throughout amplification. Competition-free amplification of phage preserves ligands that are usually lost in phage display screen. (Figure Presented). © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：5301 / 5304

页数：4

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