Stabilized, Superparamagnetic Functionalized Graphene/Fe3O4@Au Nanocomposites for a Magnetically-Controlled Solid-State Electrochemiluminescence Biosensing Application

被引：108
作者：
Gu, Wenling [1 ,2 ]
Deng, Xi [1 ]
Gu, Xiaoxiao [1 ]
Jia, Xiaofang [1 ,2 ]
Lou, Baohua [1 ,2 ]
Zhang, Xiaowei [1 ,2 ]
Li, Jing [1 ]
Wang, Erkang [1 ]
机构：
[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
来源：
ANALYTICAL CHEMISTRY | 2015年 / 87卷 / 03期
基金：
中国国家自然科学基金;
关键词：
ELECTROGENERATED CHEMILUMINESCENCE; CAPILLARY-ELECTROPHORESIS; SENSITIVE DETECTION; GOLD NANOPARTICLES; GRAPHENE OXIDE; ELECTROCHEMICAL CYTOSENSOR; SIGNAL AMPLIFICATION; SILVER NANOPARTICLES; CANCER-CELL; LUMINOL;
D O I：
10.1021/ac503966u
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Herein, a multifunctional nanoarchitecture has been developed by integrating the branched poly(ethylenimine) functionalized graphene/iron oxide hybrids (BGNs/Fe3O4) and luminol capped gold nanoparticles (luminol-AuNPs). The luminescent luminol-AuNPs as an electrochemiluminescence marker can be assembled on the nanocarrier of BGNs/Fe3O4 hybrids efficiently via the Au-N chemical bonds and electrostatic adsorption. Meanwhile, the multifunctional nanoarchitecture has been proved with excellent electron transfer, good stability, high emission intensity, etc. Furthermore, we successfully developed an ultrasensitive magnetically-controlled solid-state electrochemiluminescence (ECL) platform for label-free determination of HeLa cells using this multifunctional nanocomposite. Excellent performance of the magnetically-controlled ECL biosensing platform has been achieved including a high sensitivity for HeLa cells with a linear range from 20 to 1 x 10 (4) cells/mL, good stability, and reproducibility.
引用
收藏
页码：1876 / 1881
页数：6
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