TREM-1low is a novel characteristic for tumor-associated macrophages in lung cancer

被引：8

作者：

Zhang, Guangbo ^{[1
]}

Liu, Hongmei ^{[2
]}

Huang, Jian ^{[3
]}

Chen, Siwen ^{[2
]}

Pan, Xudong ^{[2
]}

Huang, Haitao ^{[4
]}

Wang, Ling ^{[1
,2
]}

机构：

[1] Soochow Univ, Affiliated Hosp 1, Clin Immunol Lab, Suzhou 215007, Peoples R China

[2] Soochow Univ, Affiliated Hosp 1, Dept Special Procurement Ward, Suzhou 215007, Peoples R China

[3] Soochow Univ, Affiliated Hosp 1, Dept Emergency, Suzhou 215007, Peoples R China

[4] Soochow Univ, Affiliated Hosp 1, Dept Thorac Surg, Suzhou 215007, Peoples R China

来源：

ONCOTARGET | 2016年 / 7卷 / 26期

基金：

中国国家自然科学基金;

关键词：

TREM-1; tumor-associated macrophages; tumor microenvironment; lung cancer; MYELOID CELLS-1; INFLAMMATION; EXPRESSION; RESPONSES;

D O I：

10.18632/oncotarget.9639

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Objective: To explore the expression feature and biological functions of TREM-1 on tumor-associated macrophages (TAMs) in lung cancer. Results: The levels of TREM-1 on tissue-infiltrating monocytes/macrophage from tumor nest were significantly lower than those from nonturmor tissue or peripheral blood samples. Clinical analysis indicated that the levels of TREM-1-related TAMs were significantly decreased during cancer stages progression. The tumor-bearing mouse model further confirmed that the expression of TREM-1 on TAMs was significantly decreased with tumor growth. In addition, we found the activation of TREM-1 could significantly enhance the secretion of IL-1 beta by TAM in vitro. Furthermore, T-bet but not Eomes was found to be the key transcription factor for the TREM-1 expression on monocytes/macrophage. Methods: A total of 40 patients with non-small cell lung cancer (NSCLC) were enrolled in this study. The expression characteristics of TREM-1 in blood and tissue-infiltrating monocytes/macrophage were examined by flow cytometry analysis. After the treatment of TREM-1 antibody, which is an agonist of TREM-1, cytokines secreted by TAM were then analyzed. In LLC-tumor bearing mouse model, we further investigated the dynamic expression feature of TREM-1 on macrophage with tumor growth. Moreover, we explored the transcription factor for regulating TREM-1 expression on monocyes/macrophage with wildtype, T-bet Ko or Eomes Ko mice. Conclusion: The levels of TREM-1 were remarkably decreased during tumor progression. The low expression level of TREM-1 might be a characteristic for TAMs in lung cancer.

引用

页码：40508 / 40517

页数：10

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