Fabrication of Innovative Silk/Alginate Microcarriers for Mesenchymal Stem Cell Delivery and Tissue Regeneration

被引：36
作者：
Perteghella, Sara [1 ]
Martella, Elisa [2 ,3 ]
de Girolamo, Laura [4 ]
Orfei, Carlotta Perucca [4 ]
Pierini, Michela [1 ,3 ]
Fumagalli, Valentina [1 ]
Pintacuda, Domenica Valeria [1 ]
Chlapanidas, Theodora [1 ]
Vigano, Marco [4 ]
Farago, Silvio [5 ]
Torre, Maria Luisa [1 ]
Lucarelli, Enrico [3 ]
机构：
[1] Univ Pavia, Dept Drug Sci, Viale Taramelli 12, I-27100 Pavia, Italy
[2] Univ Bologna, Dept Biomed & Neuromotor Sci DIBINEM, Via Zamboni 33, I-40126 Bologna, Italy
[3] Rizzoli Orthopaed Inst, Orthopaed & Traumatol Clin 3, Osteoarticular Regenerat Lab, Via Giulio Cesare Pupilli 1, I-40136 Bologna, Italy
[4] IRCCS Ist Ortoped Galeazzi, Via R Galeazzi 4, I-20161 Milan, Italy
[5] Innovhub, Silk Div, Stn Sperimentali Ind, Via G Colombo 83, I-20133 Milan, Italy
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2017年 / 18卷 / 09期
关键词：
microcarriers; alginate; silk fibroin; mesenchymal stem cells; regenerative medicine; musculoskeletal tissues; STROMAL-VASCULAR FRACTION; HUMAN BONE-MARROW; HUMAN ADIPOSE-TISSUE; IN-VITRO; CARTILAGE REPAIR; SILK; DIFFERENTIATION; EXPANSION; GROWTH; SCAFFOLD;
D O I：
10.3390/ijms18091829
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The aim of this study was to exploit silk fibroin's properties to develop innovative composite microcarriers for mesenchymal stem cell (MSCs) adhesion and proliferation. Alginate microcarriers were prepared, added to silk fibroin solution, and then treated with ethanol to induce silk conformational transition. Microcarriers were characterized for size distribution, coating stability and homogeneity. Finally, in vitro cytocompatibility and suitability as delivery systems for MSCs were investigated. Results indicated that our manufacturing process is consistent and reproducible: silk/alginate microcarriers were stable, with spherical geometry, about 400 m in average diameter, and fibroin homogeneously coated the surface. MSCs were able to adhere rapidly onto the microcarrier surface and to cover the surface of the microcarrier within three days of culture; moreover, on this innovative 3D culture system, stem cells preserved their metabolic activity and their multi-lineage differentiation potential. In conclusion, silk/alginate microcarriers represent a suitable support for MSCs culture and expansion. Since it is able to preserve MSCs multipotency, the developed 3D system can be intended for cell delivery, for advanced therapy and regenerative medicine applications.
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页数：18
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