Recent insights in microbial exopolysaccharide biosynthesis and engineering strategies

被引：95

作者：

Schmid, Jochen ^{[1
]}

机构：

[1] Tech Univ Munich, Chair Chem Biogen Resources, D-94315 Straubing, Germany

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2018年 / 53卷

关键词：

C-DI-GMP; POLYSACCHARIDE SYNTHESIS; BACTERIAL CELLULOSE; CHAIN-LENGTH; O-ANTIGEN; ALGINATE PRODUCTION; PROTEIN; GENES; ROLES;

D O I：

10.1016/j.copbio.2018.01.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The distinct biosynthesis pathways for microbial exopolysaccharide production provide different engineering strategies to tailor the chemical structures of the final polymers. This review focuses on the latest insights in the various pathways and identifies bottlenecks as well as promising targets for tailoring microbial polysaccharide production. The main engineering strategies includes the combinatorial assembly of glycosyltransferases and engineering of the Wzx and Wzy proteins for flipping of repeating units as well as polymerization. In the case of synthase based polysaccharides, the use of epimerases or engineering approaches of the synthase itself as well as overexpression of c-di-GMP levels is identified as one of the most promising strategies. For sucrase-based biosynthesis, the in vitro production by engineered sucrase enzymes or adjusted production conditions is shown as a very promising method.

引用

页码：130 / 136

页数：7

共 55 条

[1] Establishing a Role for Bacterial Cellulose in Environmental Interactions: Lessons Learned from Diverse Biofilm-Producing Proteobacteria [J].

Augimeri, Richard V. ;

Varley, Andrew J. ;

Strap, Janice L. .

FRONTIERS IN MICROBIOLOGY, 2015, 6

[2] Characterization of the Pseudomonas aeruginosa Glycoside Hydrolase PsIG Reveals That Its Levels Are Critical for Psi Polysaccharide Biosynthesis and Biofilm Formation [J].

Baker, Perrin ;

Whitfield, Gregory B. ;

Hill, Preston J. ;

Little, Dustin J. ;

Pestrak, Matthew J. ;

Robinson, Howard ;

Wozniak, Daniel J. ;

Howell, P. Lynne .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2015, 290 (47) :28374-28387

[3] Challenges and perspectives in combinatorial assembly of novel exopolysaccharide biosynthesis pathways [J].

Becker, Anke .

FRONTIERS IN MICROBIOLOGY, 2015, 6

[4]

Buttimer Colin, 2017, Front Microbiol, V8, P34, DOI 10.3389/fmicb.2017.00034

[5] Regulation of oxidative response and extracellular polysaccharide synthesis by a diadenylate cyclase in Streptococcus mutans [J].

Cheng, Xingqun ;

Zheng, Xin ;

Zhou, Xuedong ;

Zeng, Jumei ;

Ren, Zhi ;

Xu, Xin ;

Cheng, Lei ;

Li, Mingyun ;

Li, Jiyao ;

Li, Yuqing .

ENVIRONMENTAL MICROBIOLOGY, 2016, 18 (03) :904-922

[6]

Corbett David, 2009, F1000 Biol Rep, V1, P30, DOI 10.3410/B1-30

[7]

Cress BF, 2014, FEMS MICROBIOL REV, V38, P660, DOI [10.1111/1574-6976.12056, 10.1111/1574-69]

[8] Pivotal Roles of the Outer Membrane Polysaccharide Export and Polysaccharide Copolymerase Protein Families in Export of Extracellular Polysaccharides in Gram-Negative Bacteria [J].

Cuthbertson, Leslie ;

Mainprize, Iain L. ;

Naismith, James H. ;

Whitfield, Chris .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2009, 73 (01) :155-+

[9] Alginate-modifying enzymes: biological roles and biotechnological uses [J].

Ertesvag, Helga .

FRONTIERS IN MICROBIOLOGY, 2015, 6

[10] Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain [J].

Florea, Michael ;

Hagemann, Henrik ;

Santosa, Gabriella ;

Abbott, James ;

Micklem, Chris N. ;

Spencer-Milnes, Xenia ;

Garcia, Laura de Arroyo ;

Paschou, Despoina ;

Lazenbatt, Christopher ;

Kong, Deze ;

Chughtai, Haroon ;

Jensen, Kirsten ;

Freemont, Paul S. ;

Kitney, Richard ;

Reeve, Benjamin ;

Ellis, Tom .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (24) :E3431-E3440

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