Fluorescence quenching effects of hemoglobin on simulated tissue phantoms in the UV-Vis range

被引：9

作者：

Anand, B. S. Suresh ^{[1
]}

Sujatha, N. ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Appl Mech, Biophoton Lab, Biomed Engn Grp, Madras 600036, Tamil Nadu, India

来源：

MEASUREMENT SCIENCE AND TECHNOLOGY | 2012年 / 23卷 / 02期

关键词：

fluorescence spectroscopy; tissue-simulating phantoms; quenching; hemoglobin; absorption; IN-VIVO; POLYSTYRENE MICROSPHERES; INTRINSIC FLUORESCENCE; SCATTERING MEDIA; OPTICAL PHANTOM; TURBID MEDIA; SPECTROSCOPY; ABSORPTION; DIAGNOSIS; EXCITATION;

D O I：

10.1088/0957-0233/23/2/025502

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Tissue-model phantoms have been used in biological spectroscopy to study light propagation in a turbid medium. In these tissue models, hemoglobin (Hb) has been widely used to simulate optical absorption. The purpose of this study is to explore the use of Hb in tissue phantoms with tyrosine (Tyr) and flavin adenine dinucleotide (FAD) as fluorophores, which can affect the emission process through quenching in addition to the effects of Hb absorption. It is found that Hb has the ability to quench the Tyr fluorescence through the phenomenon of collisional quenching based on the concentrations of Hb. In addition, it is also observed that the quenching of FAD fluorescence follows a static mechanism.

引用

页数：6

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