Effect of oligosaccharides on the antioxidant, lipid and inflammatory profiles of rats with streptozotocin-induced diabetes mellitus

被引：8

作者：

Boyanov, Krasimir O. ^{[1
]}

Choneva, Mariya A. ^{[1
]}

Dimov, Ivica ^{[1
]}

Dimitrov, Iliyan, V ^{[1
]}

Vlaykova, Tatyana, I ^{[1
]}

Gerginska, Fanka D. ^{[2
]}

Delchev, Slavi D. ^{[2
]}

Hrischev, Petar, I ^{[3
]}

Georgieva, Katerina N. ^{[3
]}

Bivolarska, Anelia, V ^{[1
]}

机构：

[1] Med Univ Plovdiv, Fac Pharm, Dept Med Biochem, 15A Vasil Aprilov Blvd, Plovdiv 4002, Bulgaria

[2] Med Univ Plovdiv, Fac Med, Dept Anat Histol & Embryol, 15A Vasil Aprilov Blvd, Plovdiv 4002, Bulgaria

[3] Med Univ Plovdiv, Fac Med, Dept Physiol, 15A Vasil Aprilov Blvd, Plovdiv 4002, Bulgaria

来源：

ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES | 2022年 / 77卷 / 9-10期

关键词：

antioxidant protection; diabetes mellitus; galacto-oligosaccharides; rats; xylo-oligosaccharides; OXIDATIVE STRESS; DNA-DAMAGE; PEROXIDATION; PLASMA; TYPE-2; POWER;

D O I：

10.1515/znc-2021-0215

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Prebiotics, gut microbiota-fermentable substances, delay the development of type I diabetes. In the present study, we investigated the effect of two prebiotics (galacto-oligosaccharides and xylo-oligosaccharides) on the antioxidant protection, lipid profile, and inflammatory activity of rats with streptozotocin-induced diabetes. The following markers were studied - malondialdehyde, 8-hydroxy-2 '-deoxyguanosine, ferric reducing ability of plasma (FRAP), triacylglycerols, total cholesterol (TC), high-density lipoproteins, C-reactive protein (CRP), and interleukin-6. Diabetes was induced in male Wistar experimental rats by streptozotocin injection, while the non-diabetic controls were injected with saline. Afterward the oligosaccharides were administered orally to the experimental animals. The blood collected following the decapitation was analyzed by ELISA. A modified protocol was used only for measuring the FRAP values. The galacto-oligosaccharides and xylo-oligosaccharides lowered the malondialdehyde levels in the diabetic rats (p < 0.05). The galacto-oligosaccharides decreased the serum levels of 8-hydroxy-2 '-deoxyguanosine (p = 0.01), while the xylo-oligosaccharides increased the FRAP (p < 0.05) in the experimental animals. None of the oligosaccharides affected triacylglycerol and interleukin-6 concentrations, but the galacto-oligosaccharides decreased the TC and CRP levels in the diabetic animals. Both oligosaccharides exert a beneficial effect on the antioxidant protection of the diabetic rats, but have a minor effect on their lipid and inflammatory profiles.

引用

页码：379 / 386

页数：8

共 45 条

[1] Oxidative DNA damage and obesity in type 2 diabetes mellitus [J].

Al-Aubaidy, Hayder A. ;

Jelinek, Herbert F. .

EUROPEAN JOURNAL OF ENDOCRINOLOGY, 2011, 164 (06) :899-904

[2] Cytokine secretion in long-standing diabetes mellitus type 1 and 2: Associations with low-grade systemic inflammation [J].

Alexandraki, Krystallenia I. ;

Piperi, Christina ;

Ziakas, Panayiotis D. ;

Apostolopoulos, Nikolaos V. ;

Makrilakis, Konstantinos ;

Syriou, Vassiliki ;

Diamanti-Kandarakis, Evanthia ;

Kaltsas, Gregory ;

Kalofoutis, Anastasios .

JOURNAL OF CLINICAL IMMUNOLOGY, 2008, 28 (04) :314-321

[3] Lipid abnormalities in streptozotocin-diabetes: Amelioration by Morus indica L. cv Suguna leaves [J].

Andallu, B. ;

Kumar, A. V. Vinay ;

Varadacharyulu, N. Ch. .

INTERNATIONAL JOURNAL OF DIABETES IN DEVELOPING COUNTRIES, 2009, 29 (03) :123-128

[4] Antidiabetic and Antioxidant Activities of Ethanolic Extract of Dried Flowers of Moringa oleifera in Streptozotocin-induced Diabetic Rats [J].

Arise, Rotimi Olusanya ;

Aburo, Oluwaseun Ruth ;

Farohunbi, Samuel Tobi ;

Adewale, Adenike Adebola .

ACTA FACULTATIS MEDICAE NAISSENSIS, 2016, 33 (04) :259-271

[5]

Benzie IFF, 1999, METHOD ENZYMOL, V299, P15

[6] The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay [J].

Benzie, IFF ;

Strain, JJ .

ANALYTICAL BIOCHEMISTRY, 1996, 239 (01) :70-76

[7] Oxidative stress and cancer:: A β-catenin convergence [J].

Bowerman, B .

SCIENCE, 2005, 308 (5725) :1119-1120

[8]

Büyükkoçak S, 2000, J ENDOCRINOL INVEST, V23, P228

[9] The evaluation of altered redox status in plasma and mitochondria of acute and chronic diabetic rats [J].

Cakatay, Ufuk ;

Kayali, Refik .

CLINICAL BIOCHEMISTRY, 2006, 39 (09) :907-912

[10] Selective increases of bifidobacteria in gut microflora improve high-fat-diet-induced diabetes in mice through a mechanism associated with endotoxaemia [J].

Cani, P. D. ;

Neyrinck, A. M. ;

Fava, F. ;

Knauf, C. ;

Burcelin, R. G. ;

Tuohy, K. M. ;

Gibson, G. R. ;

Delzenne, N. M. .

DIABETOLOGIA, 2007, 50 (11) :2374-2383

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