Quantifying nitrogen uptake and translocation for mature trees: an in situ whole-tree paired 15N labeling method

被引:15
|
作者
Zhu, Feifei [1 ,2 ,3 ]
Dai, Luming [1 ,4 ]
Hobbie, Erik A. [5 ]
Qu, Yuying [1 ,3 ]
Huang, Dan [1 ,3 ]
Gurmesa, Geshere A. [1 ,3 ]
Zhou, Xulun [1 ,4 ]
Wang, Ang [1 ,2 ,3 ]
Li, Yinghua [4 ]
Fang, Yunting [1 ,2 ,3 ]
机构
[1] Chinese Acad Sci, Inst Appl Ecol, CAS Key Lab Forest Ecol & Management, Shenyang 110016, Peoples R China
[2] CERN, Qingyuan Forest, Shenyang 110016, Peoples R China
[3] Key Lab Stable Isotope Tech & Applicat, Shenyang 110016, Liaoning, Peoples R China
[4] Northeastern Univ, Sch Resources & Civil Engn, Shenyang 110819, Peoples R China
[5] Univ New Hampshire, Earth Syst Res Ctr, Morse Hall, Durham, NH 03824 USA
基金
中国国家自然科学基金;
关键词
mature larch; nitrogen uptake preference; temperate forest; SPRUCE PICEA-ABIES; INORGANIC NITROGEN; ORGANIC-NITROGEN; NUTRIENT-UPTAKE; SCOTS PINE; NITRATE ASSIMILATION; AMINO-ACIDS; ROOT UPTAKE; MASS-FLOW; AMMONIUM;
D O I
10.1093/treephys/tpab060
中图分类号
S7 [林业];
学科分类号
0829 ; 0907 ;
摘要
Nitrogen (N) is one of the major nutrients limiting plant growth in terrestrial ecosystems. To avoid plant-microbe competition, previous studies on plant N uptake preference often used hydroponic experiments on fine roots of seedlings and demonstrated ammonium preference for conifer species; however, we lack information about N uptake and translocation in the field. In this paper, we described a method of in situ paired N-15 labeling and reported the rates and time course of N uptake and translocation by mature trees in situ. We added N-15-enriched ammonium or nitrate, together with the nitrification inhibitor dicyandiamide, to paired Larix kaempferi (Lamb.) Carr (larch) trees from 30-, 40- and 50-year-old plantations. Fine roots, coarse roots, leaves and small branches were collected 2, 4, 7, 14 and 30 days after labeling. Nitrate uptake and translocation averaged 1.59 +/- 0.16 mu g N-15 g(-1) day(-1), which is slightly higher than ammonium (1.08 +/- 0.10 mu g N-15 g(-1) day(-1)), in all tree organs. Nitrate contributed 50-78% to N uptake and translocation, indicating efficient nitrate use by larch in situ. We observed no age effect. We suggest that sampling leaves after 4 days of N-15 labeling is sufficient to detect mature tree N uptake preference in situ. Whole-tree N-15-ammonium recovery equaled that of N-15-nitrate 30 days after N-15 addition, implying the importance of both ammonium and nitrate to mature larch N use in the long run. We conclude that our method is promising for studying mature tree N uptake preference in situ and can be applied to other conifer and broadleaf species. We suggest using highly enriched N-15 tracer to overcome soil dilution and a nitrification inhibitor to minimize ammonium transformation to nitrate. Our study revealed mature tree N preference in situ and demonstrated the strong contribution of nitrate toward mature larch growth on soils rich in nitrate.
引用
收藏
页码:2109 / 2125
页数:17
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