An anonymous questionnaire survey was designed to explore relationship between work-family conflict and job satisfaction. The sample (N=155) selected from an integrative hospital in Beijing. Findings: respondents reported more work-to-family conflict than family-to-work conflict; Job satisfaction correlated negatively to work-to-family conflict and family-to-work conflict, especially to the former conflict. Of all the respondents, no gender and educational level differences in work-family conflict and overall job satisfaction, as well as each single facet of job satisfaction. However, work-family conflict and job satisfaction differed in age, tenure, position, and marriage status. Overall job satisfaction and single facet of satisfaction have statistically relationship to work-family conflict. Moreover work-to-family conflict and family-to-work conflict correlated to single facet of job satisfaction differently. Indications of results to human resource management and research limitation have been discussed in the final.